Recombinant measles virus encoding the spike protein of SARS-CoV-2 efficiently induces Th1 responses and neutralizing antibodies that block SARS-CoV-2 variants

被引：4

作者：

Kwak, Hye Won ^{[1
,2
,3
]}

Park, Hyo-Jung ^{[1
,2
]}

Jung, Seo-Yeon ^{[4
]}

Oh, Eun Young ^{[5
]}

Park, Sang-In ^{[6
]}

Kim, Yeonhwa ^{[5
]}

Park, Hyeong-Jun ^{[1
,2
,3
]}

Park, Sohyun ^{[5
]}

Kim, You-Jin ^{[7
]}

Ko, Hae Li ^{[6
]}

Lee, Jung-Ah ^{[7
]}

Won, Hyeran ^{[7
]}

Hwang, Yun-Ho ^{[7
]}

Kim, Seo Yeon ^{[7
]}

Kim, Se Eun ^{[7
]}

Bae, Seoung Eun ^{[8
]}

Yoon, Minhyuk ^{[8
]}

Kim, Jae-Ouk ^{[8
]}

Song, Manki ^{[8
]}

Lee, Su Jeen ^{[4
]}

Seo, Ki-Weon ^{[4
]}

Lee, Kunse ^{[4
]}

Kim, Dokeun ^{[7
]}

Kim, Hun ^{[4
]}

Lee, Sang-Myeong ^{[5
]}

Hong, So-Hee ^{[9
]}

Nam, Jae-Hwan ^{[1
,2
,10
]}

机构：

[1] Catholic Univ Korea, Dept Med & Biol Sci, Gyeonggi Do, Bucheon, South Korea

[2] Catholic Univ Korea, BK Plus Dept Biotechnol, Gyeonggi Do, Bucheon, South Korea

[3] SML Biopharm, Gyeonggi Do, Bucheon, South Korea

[4] SK Biosci, Dept R&D, Pangyoro, South Korea

[5] Chungbuk Natl Univ, Coll Vet Med, Cheongju 28644, South Korea

[6] Scripps Korea Antibody Inst, Div Res Program, Chunchon, Kangwon Do, South Korea

[7] Natl Inst Hlth, Natl Inst Infect Dis, Ctr Vaccine Res, Div Infect Dis Vaccine Res, Sejong, South Korea

[8] Int Vaccine Inst, Sci Unit, Seoul 08826, South Korea

[9] Ewha Womans Univ, Coll Med, Dept Microbiol, Seoul 07804, South Korea

[10] Catholic Univ Korea, Dept Med & Biol Sci, 43-1 Yeokgok Dong, Bucheon 14662, South Korea

来源：

VACCINE | 2023年 / 41卷 / 11期

基金：

新加坡国家研究基金会;

关键词：

SARS-CoV-2; Measles virus vector; Neutralizing antibody; VACCINE;

D O I：

10.1016/j.vaccine.2023.02.005

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Owing to the rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its vari-ants, the development of effective and safe vaccines has become a priority. The measles virus (MeV) vac-cine is an attractive vaccine platform as it has been administered to children for more than 40 years in over 100 countries. In this study, we developed a recombinant MeV expressing the full-length SARS-CoV-2 spike protein (rMeV-S) and tested its efficacy using mouse and hamster models. In hCD46Tg mice, two-dose rMeV-S vaccination induced higher Th1 secretion and humoral responses than one-dose vacci-nation. Interestingly, neutralizing antibodies induced by one-dose and two-dose rMeV-S immunization effectively blocked the entry of the a, b, c, and d variants of SARS-CoV-2. Furthermore, two-dose rMeV-S immunization provided complete protection against SARS-CoV-2 in the hamster model. These results suggest the potential of rMeV-S as a vaccine candidate for targeting SARS-CoV-2 and its variants.(c) 2023 Elsevier Ltd. All rights reserved.

引用

页码：1892 / 1901

页数：10

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