First detection of bovine tuberculosis by Ziehl-Neelsen staining and polymerase chain reaction at dairy farms in the Lekok Sub-District, Pasuruan Regency, and Surabaya region, Indonesia

被引：0

作者：

Desire, Itfetania Aemilly ^{[1
]}

Luqman, Muhammad ^{[1
]}

Puspitasari, Yulianna ^{[2
]}

Tyasningsih, Wiwiek ^{[2
]}

Wardhana, Dhandy Koesoemo ^{[3
]}

Meles, Dewa Ketut ^{[4
]}

Dhamayanti, Yeni ^{[5
]}

Permatasari, Dian Ayu ^{[3
]}

Witaningrum, Adiana Mutamsari ^{[3
]}

Perwitasari, Agnes Dwi Sis ^{[6
]}

Raharjo, Hartanto Mulyo ^{[2
]}

Ayuti, Siti Rani ^{[7
]}

Kurniawan, Shendy Canadya ^{[8
]}

Kamaruzaman, Intan Noor Aina ^{[9
]}

Silaen, Otto Sahat Martua ^{[10
]}

机构：

[1] Univ Airlangga, Fac Vet Med, Bachelor Program Vet Med, Surabaya, E Java, Indonesia

[2] Univ Airlangga, Fac Vet Med, Div Vet Microbiol, Surabaya, E Java, Indonesia

[3] Univ Airlangga, Fac Vet Med, Div Vet Publ Hlth, Surabaya, E Java, Indonesia

[4] Univ Airlangga, Fac Vet Med, Div Basic Vet Med, Surabaya, E Java, Indonesia

[5] Univ Airlangga, Fac Vet Med, Div Vet Anat, Surabaya, E Java, Indonesia

[6] Univ Airlangga, Inst Trop Dis, Dept TB, Surabaya, E Java, Indonesia

[7] Univ Syiah Kuala, Fac Vet Med, Biochem Lab, Banda Aceh, Aceh, Indonesia

[8] Wageningen Univ & Res, Dept Anim Sci, Master Program Anim Sci, Specialisat Mol Cell & Organ Functioning, Wageningen, Netherlands

[9] Univ Malaysia Kelantan, Fac Vet Med, Dept Vet Preclin Sci, Kota Baharu, Kelantan, Malaysia

[10] Univ Indonesia, Fac Med, Doctoral Program Biomed Sci, Jakarta, Indonesia

来源：

VETERINARY WORLD | 2024年 / 17卷 / 03期

关键词：

bovine tuberculosis; cattle; polymerase chain reaction; public health; raw milk; MYCOBACTERIUM-TUBERCULOSIS; DIFFERENTIATION; COMPLEX; PREVALENCE; MICROTI; CATTLE; PCR;

D O I：

10.14202/vetworld.2024.577-584

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Background and Aim: Bovine tuberculosis (TB) is a zoonotic disease of great public health importance, particularly in Indonesia, where control measures are limited or are not implemented. This study aimed to detect the presence of Mycobacterium pathogens in milk samples from dairy cattle in Pasuruan regency and Surabaya City, East Java, using Ziehl-Neelsen acid -fast staining and polymerase chain reaction (PCR). Materials and Methods: Milk samples were aseptically collected from 50 cattle in the Lekok Subdistrict, Pasuruan Regency, and 44 from dairy farms in the Lakarsantri Subdistrict, Wonocolo Subdistrict, Mulyorejo Subdistrict, and Kenjeran Subdistrict, Surabaya, East Java. To detect Mycobacteria at the species level, each sample was assessed by Ziehl-Neelsen staining and PCR using the RD1 and RD4 genes. Results: The results of PCR assay from 50 samples in Lekok Subdistrict, Pasuruan Regency showed that 30 samples (60%) were positive for Mycobacterium tuberculosis and two samples (4%) were positive for Mycobacterium bovis, although Ziehl-Neelsen staining did not show the presence of Mycobacterium spp. In the Surabaya region, 31 samples (70.45%) were positive for M. tuberculosis and three samples (6.8%) were positive for M. bovis. Six samples (13.63%) from all PCRpositive samples could be detected microscopically with Ziehl-Neelsen. Conclusion: The presence of bovine TB in this study supports the importance of using a molecular tool alongside routine surveillance for a better understanding of the epidemiology of bovine TB in East Java.

引用

页码：577 / 584

页数：8

共 1 条

[1] Usefulness of the polymerase chain reaction dot-blot assay, used with Ziehl-Neelsen staining, for the rapid and convenient diagnosis of pulmonary tuberculosis in human immuno deficiency virus-seropositive and-seronegative individuals
Scherer, Luciene C.
Sperhacke, Rosa D.
Rossetti, Maria L. R.
Ruffino-Netto, Antonio
Kritski, Afranio L.
INFECTIOUS DISEASE REPORTS, 2011, 3 (01) : 7 - 11

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