Detection of non-small cell lung cancer marker CYFRA21-1 via Mxene-based immunoelectrochemical sensor

被引：5
作者：
Li, Dong [1 ]
Yin, Guoqiang [1 ]
Li, Zulei [1 ]
机构：
[1] Zibo Cent Hosp, Dept Thorac Surg, Zibo, Shandong, Peoples R China
来源：
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE | 2023年 / 18卷 / 03期
关键词：
Chitosan; MXene; Biotin labeling; Horseradish peroxidase; Immunosensor; CHEMOTHERAPY; BIOSENSOR; ELECTRODE; NANOCOMPOSITES;
D O I：
10.1016/j.ijoes.2023.100044
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
Chitosan (CS) provides more electrochemical active sites due to its large number of hydroxyl and amino groups. MXene can provide support sites for CS to construct novel MXene@CS composites. This work uses Ti3C2Tx@CS as a bioactive membrane. Gold nanoparticles and biotin-labeled horseradish peroxidase were made as amplifi-cation elements. The complexes formed by immunoreactivity with CYFRA21-1, streptavidin-labeled carci-noembryonic antibodies are immobilized on the Ti3C2Tx@CS. The assembled immunoelectrochemical sensor was used for the highly sensitive detection of CYFRA21-1. The results showed that the novel immunosensor exhibited a good linear range (2-20 ng/mL, R2 = 0.9941) with a detection limit of 1.7 ng/mL.
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页数：6
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