Effects of PmDOME and PmSTAT knockdown on white spot syndrome virus infection in Penaeus monodon

被引：7

作者：

Laohawutthichai, Pasunee ^{[1
,2
]}

Jatuyosporn, Thapanan ^{[1
,2
]}

Supungul, Premruethai ^{[2
,3
]}

Tassanakajon, Anchalee ^{[2
]}

Krusong, Kuakarun ^{[1
]}

机构：

[1] Chulalongkorn Univ, Fac Sci, Ctr Excellence Struct & Computat Biol, Dept Biochem, Bangkok 10330, Thailand

[2] Chulalongkorn Univ, Fac Sci, Ctr Excellence Mol Biol & Genom Shrimp, Dept Biochem, Bangkok 10330, Thailand

[3] Natl Sci & Technol Dev Agcy NSTDA, Natl Ctr Genet Engn & Biotechnol BIOTEC, Pathum Thani 12120, Thailand

来源：

SCIENTIFIC REPORTS | 2023年 / 13卷 / 01期

关键词：

BLACK TIGER SHRIMP; JAK/STAT PATHWAY; GENE; IDENTIFICATION; RESPONSES; RECEPTOR; STAT; CRUSTINPM1; EXPRESSION; BIOLOGY;

D O I：

10.1038/s41598-023-37085-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway plays an important role in antiviral immunity. This research reports the full-length DOME receptor gene in Penaeus monodon (PmDOME) and examines the effects of PmDOME and PmSTAT silencing on immune-related gene expressions in shrimp hemocytes during white spot syndrome virus (WSSV) infection. PmDOME and PmSTAT were up-regulated in shrimp hemocytes upon WSSV infection. Suppression of PmDOME and PmSTAT showed significant impacts on the expression levels of ProPO2 (melanization), Vago5 (interferon-like protein) and several antimicrobial peptides, including ALFPm3, Penaeidin3, CrustinPm1 and CrustinPm7. Silencing of PmDOME and PmSTAT reduced WSSV copy numbers and delayed the cumulative mortality caused by WSSV. We postulated that suppression of the JAK/STAT signaling pathway may activate the proPO, IFN-like antiviral cytokine and AMP production, resulting in a delay of WSSV-related mortality.

引用

页数：12

共 45 条

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