The Role of Sphingosine-1-Phosphate Receptor 2 in Mouse Retina Light Responses

被引：0

作者：

Shrestha, Abhishek P. ^{[1
]}

Stiles, Megan ^{[2
,3
,4
]}

Grambergs, Richard C. ^{[5
]}

Boff, Johane M. ^{[1
]}

Madireddy, Saivikram ^{[6
]}

Mondal, Koushik ^{[5
]}

Rajmanna, Rhea ^{[1
]}

Porter, Hunter ^{[7
]}

Sherry, David M. ^{[2
]}

Proia, Richard L. ^{[8
]}

Vaithianathan, Thirumalini ^{[1
]}

Mandal, Nawajes ^{[5
,9
]}

机构：

[1] Univ Tennessee, Hlth Sci Ctr, Coll Med, Dept Pharmacol Addict Sci & Toxicol, Memphis, TN 38163 USA

[2] Univ Oklahoma Hlth Sci Ctr, Dept Cell Biol, Oklahoma City, OK 73104 USA

[3] Univ Oklahoma Hlth Sci Ctr, Dept Neurosurg, Oklahoma City, OK 73104 USA

[4] Univ Oklahoma Hlth Sci Ctr, Dept Pharmacol Sci, Oklahoma City, OK 73104 USA

[5] Univ Tennessee, Hlth Sci Ctr, Hamilton Eye Inst, Dept Ophthalmol, Memphis, TN 38163 USA

[6] Univ Tennessee, Hlth Sci Ctr, Coll Med, Memphis, TN 38163 USA

[7] Univ Oklahoma, Hlth Sci Ctr, Dept Ophthalmol, Oklahoma City, OK 73104 USA

[8] Natl Inst Diabet Digest & Kidney Dis, Genet Dev & Dis Branch, NIH, Bethesda, MD 20892 USA

[9] Univ Tennessee, Hlth Sci Ctr, Dept Anat & Neurobiol, Memphis, TN 38163 USA

来源：

BIOMOLECULES | 2023年 / 13卷 / 12期

基金：

美国国家卫生研究院;

关键词：

sphingolipid; retina; light response; electroretinography; synaptic proteins; retinal synapses; ROD BIPOLAR CELLS; SYNAPTIC RIBBONS; B-WAVE; DEPENDENT PLASTICITY; DIURNAL CHANGES; RAT RETINA; NOGO-A; EXPRESSION; ADAPTATION; PROTEINS;

D O I：

10.3390/biom13121691

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The bioactive sphingolipid sphingosine-1-phosphate (S1P) acts as a ligand for a family of G protein-coupled S1P receptors (S1PR1-5) to participate in a variety of signaling pathways. However, their specific roles in the neural retina remain unclear. We previously showed that S1P receptor subtype 2 (S1PR2) is expressed in murine retinas, primarily in photoreceptors and bipolar cells, and its expression is altered by retinal stress. This study aims to elucidate the role of S1PR2 in the mouse retina. We examined light responses by electroretinography (ERG), structural differences by optical coherence tomography (OCT), and protein levels by immunohistochemistry (IHC) in wild-type (WT) and S1PR2 knockout (KO) mice at various ages between 3 and 6 months. We found that a- and b-wave responses significantly increased at flash intensities between 400 similar to 2000 and 4 similar to 2000 cd.s/m(2), respectively, in S1PR2 KO mice relative to those of WT controls at baseline. S1PR2 KO mice also exhibited significantly increased retinal nerve fiber layer (RNFL) and outer plexiform layer (OPL) thickness by OCT relative to the WT. Finally, in S1PR2 KO mice, we observed differential labeling of synaptic markers by immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (RT-qPCR). These results suggest a specific involvement of S1PR2 in the structure and synaptic organization of the retina and a potential role in light-mediated functioning of the retina.

引用

页数：21