Molecular Analysis of Interleukin-10 Gene for-1082 G/A Polymorphism in Breast Cancer Patients

One in every nine Pakistani women has become breast cancer patients. Genetic polymorphisms of the Interleukin-10 (IL-10) gene were studied at position -1082 (G/A) in breast cancer patients in the Pakistani population. Breast cancer patients (n= 200) were recruited from different local tertiary care hospitals along with healthy subjects (n=100) for molecular analysis of the human IL-10 gene mutation at the -1082 (G/A) position. Extraction of genomic DNA was done from patients and healthy subjects. An allele-specific polymerase chain reaction determined polymorphism at a position (-1082 G/A). PCR products were analyzed by agarose gel electrophoresis to detect polymorphism. Further analysis was done by sequencing the PCR products from some randomly selected samples. Urban locality, drinking of non-filtered water, poor hygiene, and poor socioeconomic status are the risk factors for the onset of breast cancer in the Pakistani population. Molecular analysis of the human IL-10 gene revealed that the prevalence of homozygous normal GG allele was 19%, heterozygous GA was 29% and homozygous mutant AA was 52% in breast cancer patients. However, in healthy individuals, GG allele was found in 70%, GA in 20%, and AA in 10% population. These findings proposed that the IL-10 gene (AA polymorphism) at the -1082 position is most prevalent in local breast cancer patients in Pakistan.