Development of a Novel Real-Time Quantitative PCR Method for Detection of Ilyonectria robusta, the Predominant Species Causing Ginseng Rusty Root Rot

被引:3
|
作者
Jiang, Yilin [1 ]
Jin, Hui [1 ]
Li, Xiang [2 ]
Yan, Dong [1 ]
Zhang, Yalin [1 ]
Li, Mengtao [1 ]
Gao, Jie [1 ]
Lu, Baohui [1 ]
Chen, Changqing [1 ]
Jiang, Yun [2 ]
机构
[1] Jilin Agr Univ, Coll Plant Protect, Changchun 130118, Peoples R China
[2] Jilin Agr Univ, Coll Life Sci, Changchun 130118, Peoples R China
关键词
fungi; ginseng rusty root rot; herbaceous; flowering plants; Ilyonectria robusta; molecular detection; ornamentals; pathogen detection; qPCR; MOLECULAR-DETECTION ASSAY; CYLINDROCARPON-DESTRUCTANS; DISEASE; QUANTIFICATION; DIAGNOSIS;
D O I
10.1094/PDIS-06-22-1471-RE
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Rusty root rot is the most destructive soilborne disease of ginseng caused by pathogenic Ilyonectria spp., predominantly Ilyonectria robusta, in China. However, there remains no effective strategy to control the disease. Current control of the disease requires that soil and ginseng seeds and seedlings infected with I. robusta are avoided during planting. Therefore, rapid and accurate detection of I. robusta would be indispensable in disease control programs. A one-step polymerase chain reaction (PCR) and quantitative real-time PCR (qPCR) assay was developed to detect I. robusta in ginseng seeds, roots, and soil. The species-specific primers HIS H3-F and HIS H3-R, designed based on a partial histone gene sequence of I. robusta, yielded a 268-bp product using the optimized PCR and qPCR protocol. DNA of I. robusta was detected by qPCR in all diseased soil and ginseng roots and seeds resulting from artificial inoculation and sampled from natural fields. I. robusta was detected at an abundance of 1.42 fg/mu l at 12 h postinoculation and 191.31 fg/mu l at 7 days postinoculation in ginseng roots that showed disease symptoms. In naturally infected soil sampled from ginseng fields, pathogen abundances ranging from 13.23 to 503.39 fg/mu l were detected, which were 2.04 to 11.01 times higher than those in ginseng roots. The pathogen was first detected and was more abundant on the surface of the ginseng seed coat compared with that in the seed kernel. This study provides a high-efficiency detection technique for early diagnosis of I. robusta and real-time disease prevention and control strategies.
引用
收藏
页码:1680 / 1689
页数:10
相关论文
共 50 条
  • [1] Deciphering the transcriptomic response of Ilyonectria robusta in relation to ginsenoside Rg1 treatment and the development of ginseng rusty root rot
    Li, Qiong
    Zhan, Yu
    Xu, Yonghua
    Zhang, Lianxue
    Di, Peng
    Lu, Baohui
    Chen, Changbao
    FEMS MICROBIOLOGY LETTERS, 2022, 369 (01)
  • [2] Development and Evaluation of Real-Time Quantitative PCR Assays for Detection of Phellinus noxius Causing Brown Root Rot Disease
    Liu, Tse-Yen
    Chen, Chao-Han
    Ko, Yi-Chun
    Wu, Zong-Chi
    Liao, Ting-Zhi
    Lee, Hsin-Han
    Tsai, Isheng Jason
    Chang, Tun-Tschu
    Wu, Meng-Ling
    Tsai, Jyh-Nong
    Klopfenstein, Ned B.
    Kim, Mee-Sook
    Stewart, Jane E.
    Atibalentja, Ndeme
    Brooks, Fred E.
    Cannon, Philip G.
    Farid, A. Mohd
    Hattori, Tsutomu
    Kwan, Hoi-Shan
    Lam, Regent Yau Ching
    Ota, Yuko
    Sahashi, Norio
    Schlub, Robert L.
    Shuey, Louise S.
    Tang, Alvin M. C.
    Chung, Chia-Lin
    PLANT DISEASE, 2024, 108 (11) : 3288 - 3299
  • [3] Genomic and transcriptomic analyses of Bacillus methylotrophicus NJ13 reveal a molecular response strategy combating Ilyonectria robusta causing ginseng rusty root rot
    Li, Xiang
    Zhang, Ya-Lin
    Li, Jia
    Gao, Jie
    Jiang, Yun
    Chen, Chang-Qing
    BIOLOGICAL CONTROL, 2022, 172
  • [4] Optimization of the Production and Characterization of an Antifungal Protein by Bacillus velezensis Strain NT35 and Its Antifungal Activity against Ilyonectria robusta Causing Ginseng Rusty Root Rot
    Li, Mengtao
    Tang, Hao
    Li, Zongyan
    Song, Yu
    Chen, Lin
    Ran, Chao
    Jiang, Yun
    Chen, Changqing
    FERMENTATION-BASEL, 2023, 9 (04):
  • [5] Fusarium spp. Causing Dry Rot on Potatoes in Norway and Development of a Real-Time PCR Method for Detection of Fusarium coeruleum
    Pia Heltoft
    May Bente Brurberg
    Monica Skogen
    Vinh Hong Le
    Jafar Razzaghian
    Arne Hermansen
    Potato Research, 2016, 59 : 67 - 80
  • [6] Fusarium spp. Causing Dry Rot on Potatoes in Norway and Development of a Real-Time PCR Method for Detection of Fusarium coeruleum
    Heltoft, Pia
    Brurberg, May Bente
    Skogen, Monica
    Vinh Hong Le
    Razzaghian, Jafar
    Hermansen, Arne
    POTATO RESEARCH, 2016, 59 (01) : 67 - 80
  • [7] Development of a real-time quantitative PCR method for detection and quantification of Prevotella copri
    Phebe Verbrugghe
    Olivier Van Aken
    Frida Hållenius
    Anne Nilsson
    BMC Microbiology, 21
  • [8] Development of a real-time quantitative PCR method for detection and quantification of Prevotella copri
    Verbrugghe, Phebe
    Van Aken, Olivier
    Hallenius, Frida
    Nilsson, Anne
    BMC MICROBIOLOGY, 2021, 21 (01)
  • [9] Development of a real-time PCR assay for the direct detection of Candida species causing Vulvovaginal candidiasis
    Tardif, Keith D.
    Schlaberg, Robert
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2017, 88 (01) : 39 - 40
  • [10] Development of a real-time PCR assay for detection of the Raffaelea species causing Laurel wilt disease
    Dreaden, T. J.
    Smith, J. A.
    Mayfield, A. E.
    PHYTOPATHOLOGY, 2008, 98 (06) : S48 - S48