Electrical stimulation promotes functional recovery after spinal cord injury by activating endogenous spinal cord-derived neural stem/progenitor cell: an in vitro and in vivo study

被引：9

作者：

Bang, Woo-Seok ^{[1
]}

Han, Inbo ^{[2
]}

Mun, Seul-Ah ^{[3
]}

Hwang, Jong -Moon ^{[4
]}

Noh, Sung Hyun ^{[5
]}

Son, Wonsoo ^{[3
]}

Cho, Dae-Chul ^{[3
]}

Kim, Byoung-Joon ^{[3
]}

Kim, Chi Heon ^{[6
]}

Choi, Hyuk ^{[7
]}

Kim, Kyoung-Tae ^{[3
]}

机构：

[1] Topspine Hosp, Dept Neurosurg, Daegu, South Korea

[2] CHA Univ, CHA Bundang Med Ctr, Dept Neurosurg, Seongnam Si 13496, South Korea

[3] Kyungpook Natl Univ, Kyungpook Natl Univ Hosp, Sch Med, Dept Neurosurg, Daegu, South Korea

[4] Daegu Fatima Hosp, Dept Rehabil Med, Daegu, South Korea

[5] Ajou Univ, Sch Med, Dept Neurosurg, Suwon, South Korea

[6] Seoul Natl Univ, Coll Med, Dept Neurosurg, Seoul, South Korea

[7] Korea Univ, Grad Sch Med, Dept Med Sci, Seoul, South Korea

来源：

SPINE JOURNAL | 2024年 / 24卷 / 03期

关键词：

Biphasic electrical current; Neural stem/progenitor cells; Spinal cord injury; Wnt signaling; EMBRYONIC STEM-CELLS; CORTICOSPINAL TRACT; PROGENITOR CELLS; DIFFERENTIATION; EXPRESSION; RATS; TRANSPLANTATION; NEUROGENESIS; NEURONS; NEUROTROPHINS;

D O I：

10.1016/j.spinee.2023.10.004

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

BACKGROUND CONTEXT: Electrical stimulation is a noninvasive treatment method that has gained popularity in the treatment of spinal cord injury (SCI). Activation of spinal cord -derived neural stem/progenitor cell (SC-NSPC) proliferation and differentiation in the injured spinal cord may elicit considerable neural regenerative effects. PURPOSE: This study aimed to explore the effect of electrical stimulation on the neurogenesis of SC-NSPCs. STUDY DESIGN: This study analyzed the effects of electrical stimulation on neurogenesis in rodent SC-NSPCs in vitro and in vivo and evaluated functional recovery and neural circuitry improvements with electrical stimulation using a rodent SCI model. METHODS: Rats (20 rats/group) were assigned to sham (Group 1), SCI only (Group 2), SCI + electrode implant without stimulation (Group 3), and SCI + electrode with stimulation (Group 4) groups to count total SC-NSPCs and differentiated neurons and to evaluate morphological changes in differentiated neurons. Furthermore, the Basso, Beattie, and Bresnahan scores were analyzed, and the motor- and somatosensory-evoked potentials in all rats were monitored. RESULTS: Biphasic electrical currents enhanced SC-NSPC proliferation differentiation and caused qualitative morphological changes in differentiated neurons in vitro. Electrical stimulation promoted SC-NSPC proliferation and neuronal differentiation and improved functional outcomes and neural circuitry in SCI models. Increased Wnt3, Wnt7, and b-catenin protein levels were also observed after electrical stimulation. CONCLUSIONS: Our study proved the beneficial effects of electrical stimulation on SCI. The Wnt/b-catenin pathway activation may be associated with this relationship between electrical stimulation and neuronal regeneration after SCI. CLINICAL SIGNIFICANCE: The study confirmed the benefits of electrical stimulation on SCI based on cellular, functional, electrophysiological, and histological evidence. Based on these findings, we expect electrical stimulation to make a positive and significant difference in SCI treatment strategies. (c) 2023 Published by Elsevier Inc.

引用

页码：534 / 553

页数：20

共 65 条

[1] One hour electrical stimulation accelerates functional recovery after femoral nerve repair [J].