Floating electrode-dielectric barrier discharge-based plasma promotes skin regeneration in a full-thickness skin defect mouse model

被引:0
|
作者
Subramaniam, Mohana Devi [1 ,2 ]
Bae, Joon Suk [2 ]
Son, Jiwon [1 ]
Anggradita, Laurensia Danis [1 ,3 ]
Kim, Min-Kyu [1 ]
Lee, Min Yong [1 ,3 ]
Jang, Seokyoon [4 ]
Choi, Kwangok [4 ]
Lee, Justine C. [5 ]
Nam, Seung Min [2 ]
Hwang, Yongsung [1 ,3 ]
机构
[1] Soonchunhyang Univ, Soonchunhyang Inst Medi Bio Sci SIMS, Cheonan Si 31151, South Korea
[2] Soonchunhyang Univ, Bucheon Hosp, Coll Med, Dept Plast & Reconstruct Surg, Bucheon Si, Gyeonggi Do, South Korea
[3] Soonchunhyang Univ, Dept Integrated Biomed Sci, Asan 31538, Chungnam Do, South Korea
[4] HK MnS Co Ltd, Cheongju 18111, Gyeonggi Do, South Korea
[5] Univ Calif Los Angeles, David Geffen Sch Med, Dept Surg, Div Plast & Reconstruct Surg, Los Angeles, CA 90095 USA
关键词
Wound healing; Non-thermal plasma; Collagen; Inflammation; Macrophages; MACROPHAGES; ACTIVATION;
D O I
10.1007/s13534-024-00356-5
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Wound healing involves a complex and dynamic interplay among various cell types, cytokines, and growth factors. Macrophages and transforming growth factor-beta 1 (TGF-beta 1) play an essential role in different phases of wound healing. Cold atmospheric plasma has a wide range of applications in the treatment of chronic wounds. Hence, we aimed to investigate the safety and efficacy of a custom-made plasma device in a full-thickness skin defect mouse model. Here, we investigated the wound tissue on days 6 and 12 using histology, qPCR, and western blotting. During the inflammation phase of wound repair, macrophages play an important role in the onset and resolution of inflammation, showing decreased F4/80 on day 6 of plasma treatment and increased TGF-beta 1 levels. The plasma-treated group showed better epidermal epithelialization, dermal fibrosis, collagen maturation, and reduced inflammation than the control group. Our findings revealed that floating electrode-dielectric barrier discharge (FE-DBD)-based atmospheric-pressure plasma promoted significantly faster wound healing in the plasma-treated group than that in the control group with untreated wounds. Hence, plasma treatment accelerated wound healing processes without noticeable side effects and suppressed pro-inflammatory genes, suggesting that FE-DBD-based plasma could be a potential therapeutic option for treating various wounds.
引用
收藏
页码:605 / 616
页数:12
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