Eleusine indica Cytochrome P450 and Glutathione S-Transferase Are Linked to High-Level Resistance to Glufosinate

被引：16
|
作者：
He, Sifen [1 ,2 ]
Liu, Min [3 ]
Chen, Wen [3 ]
Bai, Dingyi [3 ]
Liao, Yuxi [3 ]
Bai, Lianyang [1 ,2 ,3 ]
Pan, Lang [3 ]
机构：
[1] Hunan Univ, Coll Biol, Longping Branch, Changsha 410125, Peoples R China
[2] Hunan Acad Agr Sci, Hunan Agr Biotechnol Res Inst, Changsha 410125, Peoples R China
[3] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Peoples R China
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2023年 / 71卷 / 39期
基金：
中国国家自然科学基金;
关键词：
glutamine synthetase inhibitor; goosegrass; nontarget-site resistance (NTSR); enhanced herbicidemetabolism; MULTIPLE-HERBICIDE RESISTANCE; NITROGEN ASSIMILATION; INHIBITING HERBICIDES; GLYPHOSATE; SITE; METABOLISM; MECHANISMS; PARAQUAT;
D O I：
10.1021/acs.jafc.3c04325
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Eleusine indica has become a global nuisance weed and has evolved resistance to glufosinate. The involvement of target-site resistance (TSR) in glufosinate resistance in E. indica has been elucidated, while the role of nontarget-site resistance (NTSR) remains unclear. Here, we identified a glufosinate-resistant (R) population that is highly resistant to glufosinate, with a resistance index of 13.5-fold. Molecular analysis indicated that the resistance mechanism of this R population does not involve TSR. In addition, pretreatment with two known metabolic enzyme inhibitors, the cytochrome P450 (CYP450) inhibitor malathion and the glutathione S-transferase (GST) inhibitor 4-chloro-7-nitrobenzoxadiazole (NBD-Cl), increased the sensitivity of the R population to glufosinate. The results of subsequent RNA sequencing (RNA-seq) and quantitative real-time PCR (RT-qPCR) suggested that the constitutive overexpression of a GST gene (GSTU3) and three CYP450 genes (CYP94s and CYP71) may play an important role in glufosinate resistance. This study provides new insights into the resistance mechanism of E. indica .
引用
收藏
页码：14243 / 14250
页数：8
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