Fabrication of a selective and sensitive electro-synthesized molecularly imprinted polymer-based electrochemical sensor for the determination of xanthine

Molecularly imprinted polymer (MIP)-based electrochemical sensors have always won the attention of researchers due to their predetermined selectivity and high mechanical and chemical stability. A MIP-based voltammetric sensor for the selective determination of the clinically significant molecule xanthine based on electropolymerisation of o-phenylenediamine (o-PD) in presence of xanthine on the glassy carbon electrode surface previously modified with a conducting layer of L-serine (MIP/p(L-ser)/GCE) is reported here. Numerous experimental parameters were optimized to avail maximum performance of the sensor and under these analytical constraints, the sensor was able to selectively quantify xanthine over the linear range 1.00 x-10-(4) M-1.00 x-10(-6) M with a detection limit of 5.47 x-10(-7) M. The sensor showed excellent selectivity towards xanthine in presence of other probably coexisting species. Furthermore, studies to account for the repeatability, reproducibility, and stability of the sensor were carried out and its potential applicability was successfully verified in artificial physiological fluids and in real samples of commercially available tea and coffee powders. [GRAPHICS] .