Bna.EPF2 Enhances Drought Tolerance by Regulating Stomatal Development and Stomatal Size in Brassica napus

被引:16
作者
Jiao, Peipei [1 ,2 ]
Liang, Yuanlin [1 ]
Chen, Shaoping [1 ]
Yuan, Yang [1 ]
Chen, Yongqiang [1 ]
Hu, Honghong [1 ]
机构
[1] Huazhong Agr Univ, Key Lab Crop Genet Improvement, Hubei Hongshan Lab, Wuhan 430070, Peoples R China
[2] Tarim Univ, Coll Life Sci, Xinjiang Prod & Construct Corps Key Lab Protect &, Alar 843300, Peoples R China
基金
中国国家自然科学基金;
关键词
Bna.EPF2; Brassica napus; drought tolerance; stomatal density; stomatal dimension; water use efficiency; WATER-USE EFFICIENCY; ASYMMETRIC CELL-DIVISION; AGROBACTERIUM-MEDIATED TRANSFORMATION; LIGAND-RECEPTOR PAIRS; GENETIC MANIPULATION; SECRETORY PEPTIDE; DENSITY; ARABIDOPSIS; DIFFERENTIATION; TRANSPIRATION;
D O I
10.3390/ijms24098007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Drought stress severely affects global plant growth and production. The enhancement of plant water-use efficiency (WUE) and drought tolerance by the manipulation of the stomata is an effective strategy to deal with water shortage. However, increasing the WUE and drought tolerance by manipulation on the stomata has rarely been tested in Brassica napus. Here, we isolated Bna.EPF2, an epidermal patterning factor (EPF) in Brassica napus (ecotype Westar), and identified its role in drought performance. Bna.EPF2 overexpression lines had a reduction average of 19.02% in abaxial stomatal density and smaller stomatal pore size, leading to approximately 25% lower transpiration, which finally resulted in greater instantaneous WUE and enhanced drought tolerance. Interestingly, the reduction in stomatal density did not affect the CO2 assimilation or yield-related agronomic traits in Bna.EPF2 overexpression plants. Together with the complementation of Bna.EPF2 significantly decreasing the stomatal density of Arabidopsis epf2, and Bna.EPF2 being expressed in mature guard cells, these results suggest that Bna.EPF2 not only functions in stomatal density development, but also in stomatal dimension in Brassicas. Taken together, our results suggest that Bna.EPF2 improves WUE and drought tolerance by the regulation of stomatal density and stomatal size in Brassica without growth and yield penalty, and provide insight into the manipulation of this gene in the breeding of drought tolerant plants with increased production under water deficit conditions.
引用
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页数:16
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