LINC00460/miR-143-3p/Serpine1 in Promoting Gastric Cancer (GC) Progression

Aim: Our research was to evaluate lncRNA LINC00460 in gastric cancer development and relative mechanisms by vitro study. Materials and methods: Using RT-qPCR assay to measure LINC00460, miR-143-3p, Serpine1, Vimentin, N-cadherin and E-cadherin mRNA, Serpine1, Vimentin, N-cadherin and E-cadherin expression evaluated using WB assay. Using Edu and CCK-8 assay to evaluate cell proliferation, cell apoptosis rate were evaluated by flow cytometry. Invasion cell number and wound healing rate were measured by transwell and wound healing assay. Analysis miR-1433p and Seprine 1 correlation using Dual Luciferase Reporter. Results: With LINC00460 knockdown, gastric cancer cells' proliferation were significantly depressed with apoptosis significantly increasing (P < 0.001, respectively). Meanwhile, gastric cancer cells invasion and migration abilities were significantly depressed (P < 0.001, respectively) and miR-143-3p and E-cadherin expression were significantly up-regulation, and Serpine 1, Vimentin and N-cadherin gene and proteins expression were significantly down-regulation (P < 0.001, respectively). However, miR-143-3p inhibitor supplement, GC cells' biological activities were significantly enhanced (P < 0.001, respectively) with relative gene and proteins expressions were significantly changed (P < 0.001, respectively). By Dual Luciferase Reporter, miR-143-3p could target Serpine 1. Conclusion: lncRNA LINC00460 knockdown could depress gastric cancer cell biological activities by regulation miR-143- 3p/Serpine1 axis in vivo study.