Protective effect of polysaccharide from the artificially cultivated Sanghuangporus vaninii against H2O2-induced toxicity in vitro and in zebrafish models

PFSV-2 is a purified polysaccharide with good hypolipidemic activity from artificially cultivated Sanghuangporus vaninii. In this study, PFSV-2 was characterized by scanning electron and the Congo red test. To assess the protective effect of PFSV-2, H2O2-induced oxidant injury on L929 cells and larval zebrafish models was employed. PFSV-2 significantly reduced H2O2-induced cytotoxicity in L929 cells by decreasing intracellular ROS levels and cell apoptosis. Furthermore, PFSV-2 had an obvious protective effect against H2O2-stimulated oxidative stress in larval zebrafish by decreasing heart rate and reducing ROS generation and cells death. Additionally, PFSV-2 decreased the production of MDA and increased the activity of antioxidant enzymes SOD and CAT in both L929 cells and larval zebrafish. These results suggested that PFSV-2 may be useful as an antioxidant in the medical and cosmetic industries. & COPY; 2023 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).