Synthesis and optical characteristics of 4-styrylpyridinium dyes and their conjugates with antibody

被引：6

作者：

Efimova, Anna S. ^{[1
,2
]}

Ustimova, Mariya A. ^{[1
]}

Maksimova, Margarita A. ^{[1
,3
,4
]}

Frolova, Anastasya Yu. ^{[1
,3
]}

Martynov, Vladimir I. ^{[3
]}

Deyev, Sergey M. ^{[3
]}

Pakhomov, Alexey A. ^{[1
,3
]}

Fedorov, Yurii V. ^{[1
]}

Fedorova, Olga A. ^{[1
,2
]}

机构：

[1] Russian Acad Sci, A N Nesmeyanov Inst Organoelement Cpds, Moscow 119334, Russia

[2] D I Mendeleev Univ Chem Technol Russia, Moscow 125047, Russia

[3] Russian Acad Sci, M M Shemyakin Yu A Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

[4] M V Lomonosov Moscow State Univ, Dept Chem, Moscow 119991, Russia

来源：

MENDELEEV COMMUNICATIONS | 2023年 / 33卷 / 03期

基金：

俄罗斯科学基金会;

关键词：

fluorescent probes; covalent labeling; antibody; protein; styryl dyes; pyridinium salts; INTRAMOLECULAR CHARGE-TRANSFER; FLUORESCENT-PROBES; MITOCHONDRIA; SALTS;

D O I：

10.1016/j.mencom.2023.04.027

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Two new styryl-type dyes modified with the succinimide ester group were prepared for conjugation with antibodies. The optical characteristics of the obtained compounds in different solvents were studied, and the solvatochromic properties of these dyes were revealed. The applicability of the dyes as protein labels was demonstrated in cyto-fluorometry and fluorescence microscopy.

引用

页码：384 / 386

页数：3

共 20 条

[1] Developing of fluorescence probes based on stilbazolium salts for monitoring free radical polymerization processes. II [J].

Bajorek, A ;

Trzebiatowska, K ;

Jedrzejewska, B ;

Pietrzak, M ;

Gawinecki, R ;

Paczkowski, J .

JOURNAL OF FLUORESCENCE, 2004, 14 (03) :295-307

[2] Intramolecular Charge Transfer of Push-Pull Pyridinium Salts in the Singlet Manifold [J].

Carlotti, Benedetta ;

Consiglio, Giuseppe ;

Elisei, Fausto ;

Fortuna, Cosimo G. ;

Mazzucato, Ugo ;

Spalletti, Anna .

JOURNAL OF PHYSICAL CHEMISTRY A, 2014, 118 (20) :3580-3592

[3]

COONS AH, 1950, J EXP MED, V91, P1, DOI 10.1084/jem.91.1.1

[4]

Cunningham RE, 2010, METHODS MOL BIOL, V588, P319, DOI 10.1007/978-1-59745-324-0_31

[5] Synthesis of modified conformationally fixed tricarbocyanine dyes for conjugation with therapeutic agents [J].

Doroshenko, Irina A. ;

Aminulla, Kamilla G. ;

Azev, Viatcheslav N. ;

Kulinich, Tatiana M. ;

Vasilichin, Vladislav A. ;

Shtil, Alexander A. ;

Podrugina, Tatyana A. .

MENDELEEV COMMUNICATIONS, 2021, 31 (05) :615-617

[6] Streptavidin-biotin technology: improvements and innovations in chemical and biological applications [J].

Dundas, Christopher M. ;

Demonte, Daniel ;

Park, Sheldon .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2013, 97 (21) :9343-9353

[7] Mapping protein interactions by combining antibody affinity maturation and mass spectrometry [J].

Dyson, Michael R. ;

Zheng, Yong ;

Zhang, Cunjie ;

Colwill, Karen ;

Pershad, Kritika ;

Kay, Brian K. ;

Pawson, Tony ;

McCafferty, John .

ANALYTICAL BIOCHEMISTRY, 2011, 417 (01) :25-35

[8] A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies [J].

Eaton, Samantha L. ;

Hurtado, Maica Llavero ;

Oldknow, Karla J. ;

Graham, Laura C. ;

Marchant, Thomas W. ;

Gillingwater, Thomas H. ;

Farquharson, Colin ;

Wishart, Thomas M. .

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2014, (93) :e52099

[9] Structural changes accompanying intramolecular electron transfer: Focus on twisted intramolecular charge-transfer states and structures [J].

Grabowski, ZR ;

Rotkiewicz, K ;

Rettig, W .

CHEMICAL REVIEWS, 2003, 103 (10) :3899-4031

[10] Solvation Controlled Excited-State Planarization in a Push-Pull Pyrene Dye [J].

Guo, Yuanyuan ;

Abeywickrama, Chathura S. ;

Huo, Dayujia ;

Kong, Jie ;

Tao, Min ;

Xia, Andong ;

Pang, Yi ;

Wan, Yan .

JOURNAL OF PHYSICAL CHEMISTRY C, 2020, 124 (16) :8550-8560

← 1 2 →