Generation of a PPP1CA knockout human pluripotent stem cell line via CRISPR/Cas9

被引：1

作者：

Wang, Xiaoman ^{[1
]}

Sun, Jiaan ^{[1
]}

Fu, Chong ^{[2
]}

Liu, Chang ^{[1
]}

机构：

[1] Zhengzhou Univ, Zhengzhou Cent Hosp, Dept Emergency, 16 Tongbai North Rd, Zhengzhou 450007, Peoples R China

[2] Henan Inst Metrol, 10 Baifo Rd, Zhengzhou 450008, Peoples R China

来源：

STEM CELL RESEARCH | 2023年 / 69卷

关键词：

D O I：

10.1016/j.scr.2023.103077

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The Protein phosphatase 1 catalytic subunit alpha (PPP1CA) is a alpha subunit of the PP1 complex, which known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Here, a PPP1CA knockout human embryonic stem cell line, WAe009-A-B, was generated using the CRISPR/Cas9 system to further study the function of PPP1CA. The cell line confirmed with pluripotency, normal karyotype and differentiation potential.

引用

页数：4