Bilobalide Induces Apoptosis in 3T3-L1 Mature Adipocytes through ROS-Mediated Mitochondria Pathway

被引：6

作者：

Bu, Su ^{[1
]}

Xiong, Anran ^{[1
]}

Yang, Zhiying ^{[1
]}

Aissa-Brahim, Faycal ^{[1
]}

Chen, Ying ^{[1
]}

Zhang, Yichun ^{[2
]}

Zhou, Xunyong ^{[3
]}

Cao, Fuliang ^{[2
,4
]}

机构：

[1] Nanjing Forestry Univ, Coll Biol & Environm, Nanjing 210037, Peoples R China

[2] Nanjing Forestry Univ, Coll Forestry, Nanjing 210037, Peoples R China

[3] HC Enzyme Biotech Co Ltd, Shenzhen 518001, Peoples R China

[4] Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Peoples R China

来源：

MOLECULES | 2023年 / 28卷 / 17期

关键词：

bilobalide; mature adipocyte; apoptosis; reactive oxygen species (ROS); CELL-DEATH; INHIBITS ADIPOGENESIS; DIFFERENTIATION; PREADIPOCYTE; INFLAMMATION;

D O I：

10.3390/molecules28176410

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bilobalide exhibits numerous beneficial bioactivities, including neuroprotective, anti-inflammatory, and antioxidant activity. Our previous study demonstrated that bilobalide inhibits adipogenesis and promotes lipolysis. The dose-dependent cytotoxicity was found to be specific to the mature adipocytes only, indicating the potential for regulating apoptosis in them. Herein, we aimed to investigate the apoptotic effects of bilobalide on 3T3-L1 mature adipocytes and elucidate the underlying mechanisms thereof. Flow cytometry analysis (FACS) revealed the pro-apoptotic effects of bilobalide on these cells. Bilobalide induced early apoptosis by reducing the mitochondrial membrane potential (MMP). DNA fragmentation was confirmed using TUNEL staining. Additionally, bilobalide increased the intracellular reactive oxygen species (ROS) levels and activities of Caspases 3/9. Pre-treatment with NAC (an ROS scavenger) confirmed the role of ROS in inducing apoptosis. Moreover, bilobalide up- and down-regulated the expression of Bax and Bcl-2, respectively, at the mRNA and protein expression levels; upregulated the Bax/Bcl-2 ratio; triggered the release of cytochrome c from the mitochondria; and increased the protein expression of cleaved Caspase 3, cleaved Caspase 9, and PARP cleavage. These results support the conclusion that bilobalide induces apoptosis in mature 3T3-L1 adipocytes through the ROS-mediated mitochondrial pathway, and offers potential novel treatment for obesity.

引用

页数：17

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