Inducible Rbpms-CreERT2 Mouse Line for Studying Gene Function in Retinal Ganglion Cell Physiology and Disease

被引:3
作者
Guo, Luming [1 ,2 ]
Xie, Xiaoling [2 ]
Wang, Jing [3 ,4 ]
Xiao, Haiyan [3 ,4 ]
Li, Shuchun [2 ]
Xu, Mei [1 ,2 ]
Quainoo, Ebenezer [2 ,4 ]
Koppaka, Rithwik [2 ]
Zhuo, Jiaping [2 ]
Smith, Sylvia B. [3 ,4 ]
Gan, Lin [2 ,4 ]
机构
[1] Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Peoples R China
[2] Augusta Univ, Med Coll Georgia, Dept Neurosci & Regenerat Med, Augusta, GA 30912 USA
[3] Augusta Univ, Med Coll Georgia, Dept Cellular Biol & Anat, Augusta, GA 30912 USA
[4] Augusta Univ, James & Jean Culver Vis Discovery Inst, Med Coll Georgia, Augusta, GA 30912 USA
关键词
Cre recombinase; glaucoma; optic neuropathy; RGC and axonal degeneration; RGC development; RNA-BINDING PROTEIN; DOMINANT OPTIC ATROPHY; TRANSCRIPTION FACTORS; RECOMBINASE ACTIVITY; COMPETENCE STATE; RBPMS; MARKER; CRE; SYSTEM; LAYER;
D O I
10.3390/cells12151951
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Retinal ganglion cells (RGCs) are the sole output neurons conveying visual stimuli from the retina to the brain, and dysfunction or loss of RGCs is the primary determinant of visual loss in traumatic and degenerative ocular conditions. Currently, there is a lack of RGC-specific Cre mouse lines that serve as invaluable tools for manipulating genes in RGCs and studying the genetic basis of RGC diseases. The RNA-binding protein with multiple splicing (RBPMS) is identified as the specific marker of all RGCs. Here, we report the generation and characterization of a knock-in mouse line in which a P2A-CreER(T2) coding sequence is fused in-frame to the C-terminus of endogenous RBPMS, allowing for the co-expression of RBPMS and CreER(T2). The inducible Rbpms-CreER(T2) mice exhibited a high recombination efficiency in activating the expression of the tdTomato reporter gene in nearly all adult RGCs as well as in differentiated RGCs starting at E13.5. Additionally, both heterozygous and homozygous Rbpms-CreER(T2) knock-in mice showed no detectable defect in the retinal structure, visual function, and transcriptome. Together, these results demonstrated that the Rbpms-CreER(T2) knock-in mouse can serve as a powerful and highly desired genetic tool for lineage tracing, genetic manipulation, retinal physiology study, and ocular disease modeling in RGCs.
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页数:20
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