Integrated metabolomics and network analysis reveal changes in lipid metabolisms of tripterygium glycosides tablets in rats with collagen- induced arthritis

被引：6

作者：

Gao, Yanhua ^{[1
]}

Qian, Qi ^{[1
]}

Xun, Ge ^{[1
]}

Zhang, Jia ^{[1
]}

Sun, Shuo ^{[1
]}

Liu, Xin ^{[1
]}

Liu, Fangfang ^{[1
]}

Ge, Jiachen ^{[1
]}

Zhang, Huaxing ^{[2
]}

Fu, Yan ^{[2
]}

Su, Suwen ^{[3
]}

Wang, Xu ^{[1
]}

Wang, Qiao ^{[1
]}

机构：

[1] Hebei Med Univ, Sch Pharm, Shijiazhuang 050017, Peoples R China

[2] Hebei Med Univ, Core Facil & Ctr, Shijiazhuang 050017, Peoples R China

[3] Hebei Med Univ, Dept Pharmacol, Key Lab Pharmacol & Toxicol New Drugs, Shijiazhuang 050017, Peoples R China

来源：

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL | 2023年 / 21卷

基金：

中国国家自然科学基金;

关键词：

Tripterygium glycosides tablets; Rheumatoid arthritis; Metabolomics; Network analysis; Lipid metabolisms; EXPRESSION; FIBROSIS;

D O I：

10.1016/j.csbj.2023.02.050

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tripterygium glycosides tablets (TGT) are the commonly used preparation for rheumatoid arthritis (RA). However, the changes in TGT on RA are still unclear at the metabolic level. This study aimed to reveal the biological processes of TGT in collagen-induced arthritis (CIA) rats through integrated metabolomics and network analysis. First, the CIA model in rats was established, and the CIA rats were given three doses of TGT. Then, the endogenous metabolites in the serum from normal rats, CIA rats, and CIA rats treated with varying doses of TGT were detected by UHPLC-QTOF-MS/MS. Next, univariate and multivariate statistical analyses were performed to find the differential metabolites. Finally, differential metabolites, metabolic pathways, and hub genes were analyzed integrally to reveal the biological processes of TGT in CIA rats. The paw diameter, arthritis score, immunoglobulin G (IgG) concentration, CT image, and histological assay showed that TGT had evident therapeutic effects on CIA rats. Untargeted metabolomics revealed that TGT could ameliorate the down-regulation of lipid levels in CIA rats. Four key differential metabolites were found including LysoP(18:0), LysoPA(20:4), LysoPA(18:2), and PS(O-20:0/17:1). The glycerophospholipid metabolic pathway was perturbed in treating CIA with TGT. A total of 24 genes, including PLD1, LPCAT4, AGPAT1, and PLA2G4A, were found to be the hub genes of TGT in CIA rats. In conclusion, the integrated analysis provided a novel and holistic perspective on the biological processes of TGT in CIA rats, which could give helpful guidance for further TGT on RA. Future studies based on human samples are necessary.(c) 2023 The Author(s). Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open access article under the CC BY-NC-ND license (http://creative-commons.org/licenses/by-nc-nd/4.0/).

引用

页码：1828 / 1842

页数：15

共 54 条

[1] Relationship of serum copper and HLADR4 tissue typing to disease activity and severity in patients with rheumatoid arthritis: A cross sectional study [J].

Aldabbagh, Khalid Ahmad Omer ;

Al-Bustany, Dashty Abbas .

ANNALS OF MEDICINE AND SURGERY, 2022, 73

[2]

Ao L., 2020, J TRADIT CHIN MED SC, V7, P291, DOI 10.1016/j.jtcms.2020.07.008

[3] Correlation between lipid deposition, immune-inflammatory cell content and MHC class II expression in diffuse intimal thickening of the human aorta [J].

Bobryshev, Yuri V. ;

Andreeva, Elena R. ;

Mikhailova, Irina A. ;

Andrianova, Irina V. ;

Moisenovich, Michael M. ;

Khapchaev, Shamil ;

Agapov, Igor I. ;

Sobenin, Igor A. ;

Lusta, Konstantin A. ;

Orekhov, Alexander N. .

ATHEROSCLEROSIS, 2011, 219 (01) :171-183

[4] Metabolomics-proteomics profiles delineate metabolic changes in kidney fibrosis disease [J].

Cao, Hongxin ;

Zhang, Aihua ;

Sun, Hui ;

Zhou, Xiaohang ;

Guan, Yu ;

Liu, Qi ;

Kong, Ling ;

Wang, Xijun .

PROTEOMICS, 2015, 15 (21) :3699-3710

[5] Nutrition and Rheumatoid Arthritis in the 'Omics' Era [J].

Cassotta, Manuela ;

Forbes-Hernandez, Tamara Y. ;

Cianciosi, Danila ;

Zabaleta, Maria Elexpuru ;

Cano, Sandra Sumalla ;

Dominguez, Irma ;

Bullon, Beatriz ;

Regolo, Lucia ;

Alvarez-Suarez, Jose Miguel ;

Giampieri, Francesca ;

Battino, Maurizio .

NUTRIENTS, 2021, 13 (03) :1-25

[6] Rheumatoid Arthritis Research in the 21st Century: Limitations of Traditional Models, New Technologies, and Opportunities for a Human Biology-Based Approach [J].

Cassotta, Manuela ;

Pistollato, Francesca ;

Battino, Maurizio .

ALTEX-ALTERNATIVES TO ANIMAL EXPERIMENTATION, 2020, 37 (02) :223-242

[7]

Chen PH., 2017, WORLD LATEST MED INF, V17, P13

[8] Ultra-low-dose CT detects synovitis in patients with suspected rheumatoid arthritis [J].

Diekhoff, Torsten ;

Ulas, Sevtap Tugce ;

Poddubnyy, Denis ;

Schneider, Udo ;

Hermann, Sandra ;

Biesen, Robert ;

Burmester, Gerd R. ;

Hamm, Bernd ;

Hermann, Kay Geert .

ANNALS OF THE RHEUMATIC DISEASES, 2019, 78 (01) :31-35

[9] Plasma Metabolomics Profiling of Metabolic Pathways Affected by Major Depressive Disorder [J].

Du, Yue ;

Wei, Jinxue ;

Zhang, Zijian ;

Yang, Xiao ;

Wang, Min ;

Wang, Yu ;

Qi, Xiongwei ;

Zhao, Liansheng ;

Tian, Yang ;

Guo, Wanjun ;

Wang, Qiang ;

Deng, Wei ;

Li, Minli ;

Lin, Dongtao ;

Li, Tao ;

Ma, Xiaohong .

FRONTIERS IN PSYCHIATRY, 2021, 12

[10] Future perspective: high-throughput construction of new ultrasensitive cytokine and virion liquid chips for high-throughput screening (HTS) of anti-inflammatory drugs or clinical diagnosis and treatment of inflammatory diseases [J].

Feng, Yingzhu ;

Huang, Jiuhong ;

Qu, Chuanhua ;

Huang, Mengjun ;

Chen, Zhencong ;

Tang, Dianyong ;

Xu, Zhigang ;

Wang, Bochu ;

Chen, Zhongzhu .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2020, 412 (28) :7685-7699

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