Advancements in the loop-mediated isothermal amplification technique for the rapid detection of plant viruses in various crops

被引：3

作者：

Fu, Ruizuo ^{[1
,2
]}

Sha, Yu ^{[1
]}

Xu, Xiangbin ^{[2
]}

Liu, Song-Bai ^{[1
,2
]}

机构：

[1] Suzhou Vocat Hlth Coll, Suzhou Key Lab Med Biotechnol, Suzhou 215009, Peoples R China

[2] Hainan Univ, Sch Food Sci & Engn, Haikou 570228, Peoples R China

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2024年 / 130卷

关键词：

Loop -mediated isothermal amplification; Real-time LAMP; Crop; Viruses; Viroids; ASSAY; TOMATO; LAMP;

D O I：

10.1016/j.pmpp.2024.102229

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The key to success in crop virus management is having the ability to detect pathogens early and accurately. In the last 20 years, loop-mediated isothermal amplification (LAMP) has been widely used in plant disease detection and other fields. It has the characteristics of good specificity, high sensitivity, convenience and speed. Here, we introduce the basic principle of LAMP technology and provide its applications in crop virus detection. Finally, we show the challenges and prospects in the LAMP of crop virus detection. This review will provide a reference for the diagnosis and early prevention of crop viruses by LAMP in the future.

引用

页数：8

共 54 条

[1] Recent advances on the plant manipulation by geminiviruses [J].

Aguilar, Emmanuel ;

Gomez, Borja Garnelo ;

Lozano-Duran, Rosa .

CURRENT OPINION IN PLANT BIOLOGY, 2020, 56 :56-64

[2] A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of plum viroid I (PlVd-I) [J].

Bester, Rachelle ;

Maree, Hans J. .

JOURNAL OF VIROLOGICAL METHODS, 2022, 306

[3]

Boubourakas Iraklis N, 2022, Methods Mol Biol, V2316, P195, DOI 10.1007/978-1-0716-1464-8_17

[4] Development of colorimetric and real time loop-mediated isothermal amplification (cr-LAMP) assay for rapid detection of Wheat dwarf virus (WDV) [J].

celik, Ali ;

Morca, Ali Ferhan .

CROP PROTECTION, 2021, 149

[5] Detection ofMycobacterium kansasiiusing a combination of loop-mediated isothermal amplification (LAMP) and lateral flow biosensors [J].

Chen, Chuang ;

Lu, Jia ;

Long, Bo ;

Rao, Zhengyuan ;

Gao, Yuan ;

Wang, Weina ;

Gao, Wenfeng ;

Yang, Jun ;

Zhang, Shu .

INTERNATIONAL MICROBIOLOGY, 2021, 24 (01) :75-82

[6] CHARACTERISTICS OF MICROPLATE METHOD OF ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF PLANT-VIRUSES [J].

CLARK, MF ;

ADAMS, AN .

JOURNAL OF GENERAL VIROLOGY, 1977, 34 (MAR) :475-483

[7] Application of Loop-Mediated Isothermal Amplification in an Early Warning System for Epidemics of an Externally Sourced Plant Virus [J].

Congdon, Benjamin ;

Matson, Paul ;

Begum, Farhana ;

Kehoe, Monica ;

Coutts, Brenda .

PLANTS-BASEL, 2019, 8 (05)

[8] Role of RNA silencing in plant-viroid interactions and in viroid pathogenesis [J].

Di Serio, Francesco ;

Owens, Robert A. ;

Navarro, Beatriz ;

Serra, Pedro ;

de Alba, Angel Emilio Martinez ;

Delgado, Sonia ;

Carbonell, Alberto ;

Gago-Zachert, Selma .

VIRUS RESEARCH, 2023, 323

[9] In-field molecular diagnosis of plant pathogens: recent trends and future perspectives [J].

Donoso, A. ;

Valenzuela, S. .

PLANT PATHOLOGY, 2018, 67 (07) :1451-1461

[10] Development of an FTP-LAMP assay based on TaqMan real-time PCR and LAMP for the specific detection of Xylella fastidiosa De Donno and mulberry strains in both plants and insect vectors [J].

Elbeaino, Toufic ;

Incerti, Ornella ;

Dakroub, Hiba ;

Valentini, Franco ;

Huang, Qi .

JOURNAL OF MICROBIOLOGICAL METHODS, 2020, 175

← 1 2 3 4 5 6 →