CEA cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment

被引：0

作者：

Ma, Kuiying ^{[1
]}

Wang, Xuan ^{[2
,3
]}

Wu, Linjie ^{[2
,3
]}

Yu, Lingling ^{[1
]}

Ye, Jinhui ^{[2
,3
]}

Li, Xueling ^{[2
,3
]}

Geng, Lili ^{[1
]}

Shi, Zhongyu ^{[1
]}

Yang, Huihui ^{[1
]}

Zhang, Xijuan ^{[1
]}

Zhang, Yongjian ^{[1
]}

Wu, Shuchang ^{[1
]}

Yuan, Pengfei ^{[1
]}

Zhang, Yingchi ^{[2
,3
]}

Dong, Fang ^{[2
,3
]}

Hao, Sha ^{[2
,3
]}

Hu, Linping ^{[2
,3
]}

Wei, Wensheng ^{[4
]}

Fang, Riguo ^{[1
]}

Cheng, Tao ^{[2
,3
,5
,6
,7
]}

机构：

[1] EdiGene Inc, Life Sci Pk, Beijing 102206, Peoples R China

[2] Chinese Acad Med Sci & Peking Union Med Coll, Inst Hematol, Natl Clin Res Ctr Blood Dis, State Key Lab Expt Hematol,Haihe Lab Cell Ecosyst, Tianjin 300020, Peoples R China

[3] Chinese Acad Med Sci & Peking Union Med Coll, Blood Dis Hosp, Tianjin 300020, Peoples R China

[4] Peking Univ, Peking Res Ctr,State Key Lab Prot & Plant Gene Res, Biomed Pioneering Innovat Ctr,Sch Life Sci, Beijing Adv Innovat Ctr Genom,Peking Tsinghua Ctr, Beijing 100871, Peoples R China

[5] Chinese Acad Med Sci, Ctr Stem Cell Med, Tianjin 300000, Peoples R China

[6] Peking Union Med Coll, Dept Stem Cell & Regenerat Med, Tianjin 300000, Peoples R China

[7] Tianjin Inst Hlth Sci, Tianjin 301600, Peoples R China

来源：

ISCIENCE | 2023年 / 26卷 / 12期

基金：

中国国家自然科学基金;

关键词：

EXPANSION;

D O I：

10.1016/j.isci.2023.108561

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Hematopoietic stem cell (HSC) surface markers improve the understanding of cell identity and function. Here, we report that human HSCs can be distinguished by their expression of the CEA Cell Adhesion Molecule 5 (CEACAM5, CD66e), which serves as a marker and a regulator of HSC function. CD66e* cells exhibited a 5.5-fold enrichment for functional long term HSCs compared to CD66e- cells. CD66e*CD34*CD90*CD45RA- cells displayed robust multi-lineage repopulation and serial reconstitution ability in immunodeficient mice compared to CD66e-CD34*CD90*CD45RA-cells. CD66e expression also identified almost all repopulating HSCs within the CD34*CD90*CD45RA- population. Together, these results indicated that CEACAM5 is a marker that enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment.

引用

页数：14

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