Coexistence of tet(A) and blaKPC-2 in the ST11 hypervirulent tigecycline- and carbapenem-resistant Klebsiella pneumoniae isolated from a blood sample

被引：2
作者：
Zhu, Xiaokui [1 ]
Yue, Changwu [2 ]
Geng, Huaixin [1 ]
Song, Lingjie [1 ]
Yuan, Huiming [1 ]
Zhang, Xianqin [1 ]
Sun, Chuanyu [3 ]
Luan, Guangxin [1 ]
Jia, Xu [1 ]
机构：
[1] Chengdu Med Coll, Noncoding RNA & Drug Discovery Key Lab Sichuan Pr, Chengdu, Peoples R China
[2] Yanan Univ, Med Coll, Key Lab Microbial Drugs Innovat & Transformat, Yanan, Peoples R China
[3] Fudan Univ, Huashan Hosp, Shanghai, Peoples R China
来源：
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2023年 / 42卷 / 01期
关键词：
Klebsiella pneumoniae; Hypervirulent; Tigecycline-resistant; Carbapenem-resistant; ST11; PLASMIDS;
D O I：
10.1007/s10096-022-04512-6
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Carbapenem-resistant Klebsiella pneumoniae are distributed worldwide. This study aimed to characterize a hypervirulent tigecycline-resistant and carbapenem-resistant Klebsiella pneumoniae strain, XJ-K2, collected from a patient's blood. We tested antimicrobial susceptibility, virulence, and whole-genome sequencing (WGS) on strain XJ-K2. WGS data were used to identify virulence and resistance genes and to perform multilocus sequence typing (MLST) and phylogenetic analysis. Three novel plasmids, including a pLVPK-like virulence plasmid (pXJ-K2-p1) and two multiple resistance plasmids (pXJ-K2-KPC-2 and pXJ-K2-p3), were discovered in strain XJ-K2. The IncFII(pCRY) plasmid pXJ-K2-p3 carried the dfrA14, sul2, qnrS1, bla(LAP-2), and tet(A) resistance genes. The IncFII(pHN7A8)/IncR plasmid pXJ-K2-KPC-2 also carried a range of resistance elements, containing rmtB, bla(KPC-2), bla(TEM-1), bla(CTX-M-65), and fosA3. MLST analysis revealed that strain XJ-K2 belonged to sequence type 11 (ST11). Seven complete phage sequences and many virulence genes were found in strain XJ-K2. Meanwhile, antimicrobial susceptibility tests and G. mellonella larval infection models confirmed the extensively drug resistance (XDR) and hypervirulence of KJ-K2. To our knowledge, this is the first observation and description of the ST11 hypervirulent tigecycline- and carbapenem-resistant K. pneumoniae strain co-carrying bla(KPC-2) and the tet(A) in a patient's blood in China. Further investigation is needed to understand the resistance and virulence mechanisms of this significant hypervirulent tigecycline- and carbapenem-resistant strain.
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页码：23 / 31
页数：9
相关论文
共 30 条
[1]   Molecular Analysis of Ciprofloxacin Resistance Mechanisms in Malaysian ESBL-Producing Klebsiella pneumoniae Isolates and Development of Mismatch Amplification Mutation Assays (MAMA) for Rapid Detection of gyrA and parC Mutations [J].
Al-Marzooq, Farah ;
Yusof, Mohd Yasim Mohd ;
Tay, Sun Tee .
BIOMED RESEARCH INTERNATIONAL, 2014, 2014
[2]   Assembling large genomes with single-molecule sequencing and locality-sensitive hashing [J].
Berlin, Konstantin ;
Koren, Sergey ;
Chin, Chen-Shan ;
Drake, James P. ;
Landolin, Jane M. ;
Phillippy, Adam M. .
NATURE BIOTECHNOLOGY, 2015, 33 (06) :623-+
[3]   In Silico Detection and Typing of Plasmids using PlasmidFinder and Plasmid Multilocus Sequence Typing [J].
Carattoli, Alessandra ;
Zankari, Ea ;
Garcia-Fernandez, Aurora ;
Larsen, Mette Voldby ;
Lund, Ole ;
Villa, Laura ;
Aarestrup, Frank Moller ;
Hasman, Henrik .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2014, 58 (07) :3895-3903
[4]   Hypervirulence and hypermucoviscosity: Two different but complementary Klebsiella spp. phenotypes? [J].
Carlos Catalan-Najera, Juan ;
Garza-Ramos, Ulises ;
Barrios-Camacho, Humberto .
VIRULENCE, 2017, 8 (07) :1111-1123
[5]   Convergence of carbapenem-resistance and hypervirulence in Klebsiella pneumoniae [J].
Chen, Liang ;
Kreiswirth, Barry N. .
LANCET INFECTIOUS DISEASES, 2018, 18 (01) :2-3
[6]  
CHENG DL, 1991, ARCH INTERN MED, V151, P1557
[7]  
Chin CS, 2013, NAT METHODS, V10, P563, DOI [10.1038/NMETH.2474, 10.1038/nmeth.2474]
[8]  
Chiu SK, 2017, ANTIMICROB AGENTS CH, V61, DOI [10.1128/AAC.00391-17, 10.1128/aac.00391-17]
[9]   Evolution of Carbapenem-Resistant Serotype K1 Hypervirulent Klebsiella pneumoniae by Acquisition of blaVIM-1-Bearing Plasmid [J].
Dong, Ning ;
Sun, Qiaoling ;
Huang, Yonglu ;
Shu, Lingbin ;
Ye, Lianwei ;
Zhang, Rong ;
Chen, Sheng .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2019, 63 (09)
[10]   Carriage of blaKPC-2 by a virulence plasmid in hypervirulent Klebsiella pneumoniae [J].
Dong, Ning ;
Lin, Dachuan ;
Zhang, Rong ;
Chan, Edward Wai-Chi ;
Chen, Sheng .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2018, 73 (12) :3317-3321
123