Fine mapping and validation of a stable QTL for thousand-kernel weight in wheat (Triticum aestivum L.)

被引：4

作者：

Meng, Deyuan ^{[1
,7
]}

Batool, Aamana ^{[1
,7
]}

Xuan, Yazhou ^{[2
]}

Pan, Ruiqing ^{[1
]}

Zhang, Na ^{[1
]}

Zhang, Wei ^{[1
]}

Zhi, Liya ^{[1
,7
]}

Ren, Xiaoli ^{[1
]}

Li, Wenqing ^{[2
]}

Li, Jijie ^{[2
]}

Niu, Yanxiao ^{[2
]}

Zheng, Shuzhi ^{[2
,6
]}

Ji, Jun ^{[1
,4
]}

Shi, Xiaoli ^{[4
]}

Wang, Lei ^{[1
,4
,6
]}

Ling, Hongqing ^{[4
,7
]}

Zhao, Chunhua ^{[5
]}

Cui, Fa ^{[5
]}

Liu, Xigang ^{[2
,6
]}

Li, Junming ^{[1
,2
,6
]}

Song, Liqiang ^{[3
,6
]}

机构：

[1] Chinese Acad Sci, Innovat Acad Seed Design, Ctr Agr Resources Res, Inst Genet & Dev Biol, Shijiazhuang 050022, Hebei, Peoples R China

[2] Hebei Normal Univ, Coll Life Sci, Hebei Res Ctr Basic Discipline Cell Biol, Key Lab Mol & Cellular Biol,Minist Educ,Hebei Key, Shijiazhuang 050024, Hebei, Peoples R China

[3] Hebei Agr Univ, Coll Agron, State Key Lab North China Crop Improvement & Regul, Baoding, Peoples R China

[4] Chinese Acad Sci, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China

[5] Ludong Univ, Univ Shandong, Sch Agr, Key Lab Mol Module Based Breeding High Yield & Abi, Yantai 264025, Shandong, Peoples R China

[6] Hebei Collaborat Innovat Ctr Cell Signaling, Shijiazhuang 050024, Hebei, Peoples R China

[7] Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China

来源：

CROP JOURNAL | 2023年 / 11卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Wheat; Thousand-kernel weight; Fine mapping; Candidate gene; QUANTITATIVE TRAIT LOCUS; GENOME-WIDE ASSOCIATION; BREAD WHEAT; GRAIN WEIGHT; AGRONOMIC TRAITS; HEXAPLOID WHEAT; CANDIDATE GENES; YIELD; SIZE; LENGTH;

D O I：

10.1016/j.cj.2023.03.007

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Thousand-kernel weight (TKW) is a measure of grain weight, a target of wheat breeding. The object of this study was to fine-map a stable quantitative trait loci (QTL) for TKW and identify its candidate gene in a recombinant inbred line (RIL) population derived from the cross of Kenong 9204 (KN9204) and Jing 411 (J411). On a high-density genetic linkage map, 24, 26 and 25 QTL were associated with TKW, kernel length (KL), and kernel width (KW), respectively. A major and stable QTL, QTkw-2D, was mapped to an 8.3 cM interval on chromosome arm 2DL. By saturation of polymorphic markers in its target region, QTkw-2D was confined to a 9.13 Mb physical interval using a secondary mapping population derived from a residually heterozygous line (F6:7). This interval was further narrowed to 2.52 Mb using QTkw2D near-isogenic lines (NILs). NILsKN9204 had higher fresh and dry weights than NILsJ411 at various grain-filling stages. The TKW and KW of NILsKN9204 were much higher than those of NILsJ411 in field trials. By comparison of both DNA sequence and expression between KN9204 and J411, TraesCS2D02G460300.1 (TraesKN2D01HG49350) was assigned as a candidate gene for QTkw-2D. This was confirmed by RNA sequencing (RNA-seq) of QTkw-2D NILs. These results provide the basis of map-based cloning of QTkw2D, and DNA markers linked to the candidate gene may be used in marker-assisted selection. (c) 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NCND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

引用

页码：1491 / 1500

页数：10