Shrimp laccase degrades polycyclic aromatic hydrocarbons from an oil spill disaster in Brazil: A tool for marine environmental bioremediation

被引：8
作者：
da Rocha, Amanda Barbosa [1 ,2 ]
Saraiva, Rogerio de Aqumo [2 ,7 ]
de Siqueira, Virginia Medeiros [5 ]
Yogui, Gilvan Takeshi [3 ]
Bezerra, Ranilson de Souza [4 ]
de Assis, Caio Rodrigo Dias [4 ]
Sousa, Mariana Sefora Bezerra [6 ]
Buarque, Diego de Souza [1 ,2 ]
机构：
[1] Univ Fed Rural Pernambuco, Unidade Acad Serra Talhada, Fazenda Saco S-N, BR-55608680 Serra Talhada, PE, Brazil
[2] UFRPE UAST, Programa Posgrad Prod Vegetal, BR-55608680 Serra Talhada, PE, Brazil
[3] Univ Fed Pernambuco, Dept Oceanog, BR-50740550 Recife, PE, Brazil
[4] Univ Fed Pernambuco, Dept Bioquim, BR-50670901 Recife, PE, Brazil
[5] Univ Fed Rural Pernambuco, Dept Biol, BR-52171900 Recife, PE, Brazil
[6] Inst Fed Ceara, Campus Iguatu, BR-63500000 Iguatu, Ceara, Brazil
[7] Univ Fed Cariri, Campus Brejo Santo, BR-63048080 Brejo Santo, Brazil
来源：
MARINE POLLUTION BULLETIN | 2023年 / 194卷
关键词：
Biotechnology; Enzyme; Litopenaeus vannamei; PAHs; BIOCHEMICAL-CHARACTERIZATION; PURIFICATION; OXIDATION; BIODEGRADATION; PROTEIN; ENZYMES; PAHS;
D O I：
10.1016/j.marpolbul.2023.115445
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Our work aims to purify, characterize and evaluate a laccase from by-products of the shrimp farming industry (Litopenaeus vannamei) for the degradation of Polycyclic Aromatic Hydrocarbons (PAHs) from 2019 oil spill in Brazilian coast. The enzyme was purified by affinity chromatography and characterized as thermostable, with activity above 90 degrees C and at alkaline pH. In addition, the laccase was also tolerant to copper, lead, cadmium, zinc, arsenic, hexane and methanol, with significant enzymatic activation in acetone and 10 mM mercury. Concerning PAHs' degradation, the enzyme degraded 42.40 % of the total compounds, degrading >50 % of fluorene, C4naphthalenes, C3-naphthalenes, C2-naphthalenes, anthracene, acenaphthene, 1-methylnaphthalene and 2-methylnaphthalene. Thus, this laccase demonstrated important characteristics for bioremediation of marine environments contaminated by crude oil spills, representing a viable and ecological alternative for these purposes.
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页数：11
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