Pilot evaluation of a single oral fecal microbiota transplantation for canine atopic dermatitis

被引:10
作者
Sugita, Koji [1 ,2 ]
Shima, Ayaka [3 ]
Takahashi, Kaho [1 ]
Ishihara, Genki [3 ]
Kawano, Koji [4 ,5 ]
Ohmori, Keitaro [1 ,6 ]
机构
[1] Tokyo Univ Agr & Technol, Grad Sch Agr, Cooperat Div Vet Sci, Tokyo, Japan
[2] Sugita Anim Hosp, Saitama, Japan
[3] Anicom Specialty Med Inst Inc, Tokyo, Japan
[4] Tokyo Anim Allergy Ctr, Tokyo, Japan
[5] Fujita Hlth Univ, Dept Gastroenterol & Gastroenterol Oncol, Toyoake, Aichi, Japan
[6] Tokyo Univ Agr & Technol, Inst Agr, Div Anim Life Sci, Tokyo, Japan
关键词
CLOSTRIDIUM-DIFFICILE INFECTION; SEVERITY; SCALE; SILVA; TOOL; DOG;
D O I
10.1038/s41598-023-35565-y
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The gut microbiota has been suggested to be involved in the pathogenesis of canine atopic dermatitis (cAD). However, the gut microbiota has not been well characterized in dogs with atopic dermatitis (AD). In addition, the efficacy of fecal microbiota transplantation (FMT) in dogs with AD remains unclear. This research, therefore, aimed to characterize the gut microbiota of dogs with AD and conduct pilot evaluation of the efficacy of a single oral FMT on clinical signs and the gut microbiota of dogs with AD. For these purposes, we used 12 dogs with AD and 20 healthy dogs. The 16S rRNA analysis of the fecal microbiota revealed significant differences between 12 dogs with AD and 20 healthy dogs. Next, a single oral FMT was performed in 12 dogs with AD as a single-arm, open-label clinical trial for 56 days. A single oral FMT significantly decreased Canine Atopic Dermatitis Extent and Severity Index (CADESI)-04 scores from day 0 (median score, 16.5) to day 56 (8) and Pruritus Visual Analog Scale (PVAS) scores from days 0 (median score, 3) to day 56 (1). Furthermore, a single oral FMT changed the composition of the fecal microbiota of dogs with AD at the phylum and genus levels. The number of common amplicon sequence variants in the fecal microbiota between donor dogs and dogs with AD was positively correlated with CADESI-04 and PVAS reduction ratios 56 days after FMT. Our findings suggest that the gut microbiota plays a pivotal role in the pathogenesis of cAD, and that oral FMT could be a new therapeutic approach targeting the gut microbiota in cAD.
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页数:11
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