Synthesis, Characterization and HPLC Analysis of Streptomycin with its Derivatives

A new streptomycin derivative was synthesized and identified using UV-Vis, FTIR, and HNMR; the streptomycin first derivative has a moderate inhibition Escherichia coli compared to the standard medication, which did not give any inhibition to this type of bacteria. A sensitive, accurate RP-HPLC method was developed to separate these streptomycin derivatives from the standard streptomycin drug and applied for the drug analysis in pharmaceuticals at optimal separation conditions using the Nucludar C18-DB column. The results showed good separation between the standard streptomycin drug and its prepared derivatives; the first, second, and third derivatives have t(R) of 2.102, 6.19, and 7.695 min, respectively, while the standard streptomycin appeared at t(R) of 5.352 min. This method was applied for the separation and estimation of streptomycin in ABBOTT and TROGE pharmaceuticals via standard graph, and the results proved that this method is accurate and the obtained concentration values match what was proven by the drug manufacturer.