Evaluation of the acute toxicity and antidiabetic activity of Coutoubea spicata "nicolau" ethanolic extract and ethyl acetate fraction

被引：0

作者：

dos Santos, Jymmys Lopes ^{[1
]}

Silva, Ana Mara de Oliveira e ^{[2
]}

Souza, Lucio Marques VIeira ^{[1
]}

Pereira, Raquel Oliveira ^{[2
]}

Lima, Clesio Andrade ^{[1
]}

Abreu, Lucas Silva ^{[4
]}

Montalvao, Monalisa Martins ^{[5
]}

Correa, Cristiane Bani ^{[5
]}

Rabelo, Thallita Kelly ^{[6
]}

Duarte, Marcelo Cavalcante ^{[7
]}

Aidar, Felipe Jose ^{[6
,8
]}

Tavares, Josean Fechine ^{[3
]}

Estevam, Charles dos Santos ^{[1
]}

Marcal, Anderson Carlos ^{[5
]}

机构：

[1] Univ Fed Sergipe, Dept Physiol, Lab Nat Prod Chem & Biochem, Sao Cristovao, Brazil

[2] Univ Fed Sergipe, Dept Nutr, Sao Cristovao, Brazil

[3] Univ Fed Paraiba, Multiuser Lab Characterizat & Anal LMCA, Joao Pessoa, Brazil

[4] Univ Fed Sergipe, Dept Morphol, Sao Cristovao, Brazil

[5] Univ Fed Sergipe, Dept Physiol, Sao Cristovao, Brazil

[6] Univ Fed Sergipe, Dept Pharm, Sao Cristovao, Brazil

[7] Univ Fed Mato Grosso, Fac Med, Posgrad Program Hlth Sci, Cuiaba, Brazil

[8] Univ Fed Sergipe, Dept Phys Educ, Sao Cristovao, Brazil

来源：

BOLETIN LATINOAMERICANO Y DEL CARIBE DE PLANTAS MEDICINALES Y AROMATICAS | 2023年 / 22卷 / 05期

关键词：

Coutoubea spicata; Acute toxicity; Diabetes; Redox profile; HPLC-ESI-MS; MS; ANTIOXIDANT ACTIVITY; SWERTIAMARIN; LITTORALE; APOPTOSIS; FLAVONOL;

D O I：

10.37360/blacpma.23.22.5.44

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

In order to understand antidiabetic potential and toxicity, this study aimed to evaluate the acute toxicity and antidiabetic activity of ethanolic extract and ethyl acetate fraction obtained from Coutoubea spicata "nicolau" shoots. Chemical constituents and acute toxicity were investigated. In alloxan-induced diabetic rats, extract and fraction were tested at dose of 100 mg/kg, p.o. Body weight gain, glucose, lipid profile and oxidative stress markers in serum and tissues were determined. In vitro antioxidant activity was performed. Swertiamarin, gentiopicrin, deoxyloganic acid, clovin and robinin, and their p-coumaric ester were identified. Extract and fraction were classified as safe (category 5). In diabetic rats, Coutoubea spicata reduced glycaemia, which was accompanied by body weight recovery gain and attenuation in oxidative stress markers. Fraction showed scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical (ABTS) radicals and reducing power higher than that of the extract. Extract and fraction of Coutoubea spicata didn't present significant toxicity and it can be investigated as a therapeutic alternative in diabetes.

引用

页码：607 / 627

页数：21

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