κ-Carrageenan/konjac glucomannan composite hydrogel-based 3D porcine cultured meat production

被引:15
作者
Gu, Xin [1 ,2 ,3 ]
Hua, Shiyuan [5 ,6 ]
Huang, Yuqin [1 ,2 ,3 ]
Liu, Shiqi [1 ,2 ,3 ]
Wang, Yizhen [1 ,2 ,3 ]
Zhou, Min [4 ,5 ,6 ,7 ]
Shan, Tizhong [1 ,2 ,3 ]
机构
[1] Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China
[2] Zhejiang Univ, Key Lab Mol Anim Nutr, Minist Educ, Hangzhou 310058, Peoples R China
[3] Key Lab Anim Feed & Nutr Zhejiang Prov, Hangzhou 310058, Peoples R China
[4] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Infect Dis, Hangzhou 310009, Peoples R China
[5] Zhejiang Univ, Sch Med, Zhejiang Univ Univ Edinburgh Inst ZJU UoE Inst, Haining, Peoples R China
[6] Zhejiang Univ, Inst Translat Med, Hangzhou 310029, Peoples R China
[7] Zhejiang Univ, Binjiang Inst, Res Ctr Life Sci & Human Hlth, Hangzhou 310053, Peoples R China
基金
中国国家自然科学基金;
关键词
Cultured fat; Scaffolds; K-Carrageenan; Konjac glucomannan; Subcutaneous pre-adipocytes; Fibro-adipogenic progenitors; LOW-FAT; KONJAC; CHALLENGES; QUALITY; PORK; BIOMATERIALS; COMBINATION; MECHANISMS; REPLACER; MATRIX;
D O I
10.1016/j.foodhyd.2024.109765
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Alternative technology for cultured meat production holds the potential to alleviate ethical, environmental, and public health concerns stemming from conventional husbandry. As a key food component, fat plays a crucial role in the sensory characteristics and nutritional value of meat products. However, current adipose tissue engineering protocols have so far proven unsuitable for edible cultured fat production. In this study, hybrid hydrogels based on Kappa-Carrageenan (Car) and konjac glucomannan (KGM) were designed as a culture scaffold for adipocytes. The edible hydrogel with excellent biocompatibility, food safety, and low cost was demonstrated to support cell proliferation and allow the formation of tissue-like cell spheres. Furthermore, both porcine subcutaneous pre-adipocytes (PreAs) and fibro-adipogenic progenitors (FAPs) acquired mature adipocyte characteristics and higher lipid accumulation in Car-KGM (CK) hydrogel, suggesting its superior ability to support in vitro adipogenesis than conventional monolayer culture and alginate (Alg)-based 3D culture. Taken together, the excellent nature of CK hydrogel makes it a versatile platform for improving the molecular and cellular phenotypes of a variety of adipocyte models. The established spheroid model opens possibilities for broad applications in cultivated fat and represents a viable strategy for the manufacture of 'snowflake pork'.
引用
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页数:16
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