Clinical Characteristics, Antimicrobial Resistance, Virulence Genes and Multi-Locus Sequence Typing of Non-Typhoidal Salmonella Serovar Typhimurium and Enteritidis Strains Isolated from Patients in Chiang Mai, Thailand

被引：7

作者：

Buddhasiri, Songphon ^{[1
]}

Sukjoi, Chutikarn ^{[2
]}

Tantibhadrasapa, Arishabhas ^{[2
]}

Mongkolkarvin, Panupon ^{[2
]}

Boonpan, Pattarapon ^{[2
]}

Pattanadecha, Thanakorn ^{[2
]}

Onton, Nattamon ^{[2
]}

Laisiriroengrai, Touch ^{[2
]}

Coratat, Sunatcha ^{[2
]}

Khantawa, Banyong ^{[3
]}

Tepaamorndech, Surapun ^{[4
]}

Duangsonk, Kwanjit ^{[2
]}

Thiennimitr, Parameth ^{[2
,5
,6
]}

机构：

[1] Chiang Mai Univ, Fac Vet Med, Dept Vet Biosci & Vet Publ Hlth, Chiang Mai 50100, Thailand

[2] Chiang Mai Univ, Fac Med, Dept Microbiol, Chiang Mai 50200, Thailand

[3] Chiang Mai Univ, Maharaj Nakorn Chiang Mai Hosp, Fac Med, Diagnost Lab, Chiang Mai 50200, Thailand

[4] Chulalongkorn Univ, Fac Med, Dept Microbiol, Bangkok 10330, Thailand

[5] Chiang Mai Univ, Res Ctr Microbial Divers & Sustainable Utilizat, Chiang Mai 50200, Thailand

[6] Chiang Mai Univ, Fac Med, Ctr Multidisciplinary Technol Adv Med, Chiang Mai 50200, Thailand

来源：

MICROORGANISMS | 2023年 / 11卷 / 10期

关键词：

non-typhoidal salmonellosis; acute gastroenteritis; multidrug-resistant Salmonella; clinical isolated Salmonella; ENTERICA; PREVALENCE; IDENTIFY; DISEASE; HUMANS; PORK;

D O I：

10.3390/microorganisms11102425

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Non-typhoidal salmonellosis (NTS) caused by ingesting Salmonella enterica contaminated food or drink remains a major bacterial foodborne disease. Clinical outcomes of NTS range from self-limited gastroenteritis to life-threatening invasive NTS (iNTS). In this study, we isolated Salmonella spp. from the stool and blood of patients hospitalized at Maharaj Nakorn Chiang Mai Hospital, Chiang Mai, Thailand, between 2016-2021 (a total of 395 cases). Then, serovar Typhimurium and Enteritidis were identified and further characterized by multiplex PCR, and multi-locus sequence typing. Our data show that multidrug resistance (MDR) sequence type 34 (ST34) and ST11 are the predominant sequence types for serovars Typhimurium and Enteritidis, respectively. Most S. Typhimurium ST34 lacks spvB, and most S. Enteritidis ST11 harbor sseI, sodCI, rpoS and spvB genes. NTS can be found in a wide range of ages, and anemia could be a significant factor for S. Typhimurium infection (86.3%). Both S. Typhimurium (6.7%) and S. Enteritidis (25.0%) can cause iNTS in immunocompromised patients. S. Typhimurium conferred MDR phenotype higher than S. Enteritidis with multiple antibiotic resistance indexes of 0.22 and 0.04, respectively. Here, we characterized the important S. Typhimurium, S. Enteritidis, and human clinical factors of NTS within the region.

引用

页数：16

共 64 条

[1] Extensive Drug-Resistant Salmonella enterica Isolated From Poultry and Humans: Prevalence and Molecular Determinants Behind the Co-resistance to Ciprofloxacin and Tigecycline [J].