The Variations' in Genes Encoding TIM-3 and Its Ligand, Galectin-9, Influence on ccRCC Risk and Prognosis

被引：4

作者：

Andrzejczak, Anna ^{[1
]}

Tupikowski, Krzysztof ^{[2
]}

Tomkiewicz, Anna ^{[1
]}

Malkiewicz, Bartosz ^{[3
]}

Ptaszkowski, Kuba ^{[4
]}

Domin, Aleksandra ^{[1
]}

Szydelko, Tomasz ^{[3
]}

Karabon, Lidia ^{[1
]}

机构：

[1] Polish Acad Sci, Hirszfeld Inst Immunol & Expt Therapy, Dept Expt Therapy, Lab Genet & Epigenet Human Dis, PL-53114 Wroclaw, Poland

[2] Lower Silesian Ctr Oncol Pulmonol & Hematol, Subdiv Urol, PL-53413 Wroclaw, Poland

[3] Wroclaw Med Univ, Univ Ctr Excellence Urol, Dept Minimally Invas & Robot Urol, PL-50556 Wroclaw, Poland

[4] Wroclaw Med Univ, Dept Clin Biomech & Physiotherapy Motor Syst Disor, PL-50556 Wroclaw, Poland

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2023年 / 24卷 / 03期

关键词：

TIM-3; HAVCR2; galectin-9 (LGALS9); clear cell renal cell carcinoma (ccRCC); single-nucleotide gene polymorphism (SNP); immunological checkpoint; disease risk; overall survival; T-CELL IMMUNOGLOBULIN; EXPRESSION; POLYMORPHISMS; SUSCEPTIBILITY; DISEASE; CANCER; DISEQUILIBRIUM; ASSOCIATION; VARIANTS; SURVIVAL;

D O I：

10.3390/ijms24032042

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Renal cell cancer is the most common type of kidney cancer in adults, and clear cell renal cell carcinoma (ccRCC) is the most diagnosed type. T cell immunoglobulin and mucin-domain-containing-3 (TIM-3) belongs to immunological checkpoints that are key regulators of the immune response. One of the known TIM-3 ligands is galectin-9 (LGALS9). A limited number of studies have shown an association between TIM-3 polymorphisms and cancer risk in the Asian population; however, there is no study on the role of LGALS9 polymorphisms in cancer. The present study aimed to analyze the influence of TIM-3 and LGALS9 polymorphisms on susceptibility to ccRCC and patient overall survival (OS), with over ten years of observations. Using TaqMan probes, ARMS-PCR, and RFPL-PCR, we genotyped two TIM-3 single-nucleotide polymorphisms (SNPs): rs1036199 and rs10057302, and four LGALS9 SNPs: rs361497, rs3751093, rs4239242, and rs4794976. We found that the presence of the rs10057302 A allele (AC + AA genotypes) as well as the rs4794976 T allele (GT + TT genotypes) decreased susceptibility to ccRCC by two-fold compared to corresponding homozygotes. A subgroup analysis showed the association of some SNPs with clinical features. Moreover, TIM-3 rs1036199 significantly influenced OS. Our results indicate that variations within TIM-3 and LGALS9 genes are associated with ccRCC risk and OS.

引用

页数：18