Attenuation of mercury phytotoxicity with a high nutritional level of nitrate in alfalfa plants grown hydroponically

Mercury (Hg) is one of the most dangerous pollutant heavy metals to the environment, which causes several toxic effects in plants upon accumulation, such as induction of oxidative stress. Nitrate (NO3-) is the prevalent form to - incorporate nitrogen (N) in higher plants, through its reduction to nitrite (NO2 (NR). We studied the physiological alterations caused by Hg (0, 6 and 30 mu M) in alfalfa plants grown at two - different levels of NO3 : low, (2 mM; LN), and high (12 mM; HN) for one week using a semi-hydroponic culture system. Several parameters of oxidative stress such as lipid peroxidation, chlorophyll content, biothiol concen-tration, and ascorbate peroxidase (APX) and glutathione reductase (GR) activities showed that HN plants were less affected by Hg. Nitrate reductase activity and NO3- concentration were also altered under Hg stress, with - - lower impact in plants nourished with high NO3 . Our results highlight the importance of the NO3 nutritional status to improve tolerance to toxic metals like Hg. ) by the enzyme nitrate reductase