The Impact of Blue Light Irradiation on Keratinocytes in Vitro

Background: This study examined the effects of irradiation with blue light on HaCaT keratinocytes. As irradiation with blue light is known to be antimicrobial, it offers a promising alternative therapy for contaminated wounds. There is evidence that red light promotes wound healing, but the potential benefits of irradiation with blue light have not yet been adequately investigated. Methods: The rate of wound closure in sterile and contaminated cells was measured using an in vitro scratch assay wound-healing model. Additionally, cell viability after treatment was determined using a Sulforhodamine B (SRB) assay. Results: In both the sterile and contaminated groups, treated cells showed delayed wound closure when compared with cells not irradiated with blue light. Additionally, treatment with blue light resulted in poorer viability in the treatment groups. Conclusion: Although irradiation with blue light may offer a promising alternative therapy for reducing bacterial colonization, our data indicate that re- epithelization may be negatively influenced by blue light. Further research is needed to clarify possible wound healing applications.