The Effects of Dietary Silybin Supplementation on the Growth Performance and Regulation of Intestinal Oxidative Injury and Microflora Dysbiosis in Weaned Piglets

被引:9
作者
Cai, Long [1 ]
Gao, Ge [1 ]
Yin, Chenggang [1 ]
Bai, Rong [1 ]
Li, Yanpin [1 ]
Sun, Wenjuan [1 ]
Pi, Yu [1 ]
Jiang, Xianren [1 ]
Li, Xilong [1 ]
机构
[1] Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Inst Feed Res, Key Lab Feed Biotechnol, Beijing 100081, Peoples R China
关键词
silybin; growth performance; intestinal health; microflora dysbiosis; mitochondria function; weaned piglet; NUTRIENT DIGESTIBILITY; SILYMARIN; ANTIOXIDANT; MITOCHONDRIA; QUALITY;
D O I
10.3390/antiox12111975
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Oxidative stress is the major incentive for intestinal dysfunction in weaned piglets, which usually leads to growth retardation or even death. Silybin has caught extensive attention due to its antioxidant properties. Herein, we investigated the effect of dietary silybin supplementation on growth performance and determined its protective effect on paraquat (PQ)-induced intestinal oxidative damage and microflora dysbiosis in weaned piglets. In trial 1, a total of one hundred twenty healthy weaned piglets were randomly assigned into five treatments with six replicate pens per treatment and four piglets per pen, where they were fed basal diets supplemented with silybin at 0, 50, 100, 200, or 400 mg/kg for 42 days. In trial 2, a total of 24 piglets were randomly allocated to two dietary treatments with 12 replicates per treatment and 1 piglet per pen: a basal diet or adding 400 mg/kg silybin to a basal diet. One-half piglets in each treatment were given an intraperitoneal injection of paraquat (4 mg/kg of body weight) or sterile saline on day 18. All piglets were euthanized on day 21 for sample collection. The results showed that dietary supplementation with 400 mg/kg silybin resulted in a lower feed conversion ratio, diarrhea incidence, and greater antioxidant capacity in weaned piglets. Dietary silybin enhanced intestinal antioxidant capacity and mitochondrial function in oxidative stress piglets induced by PQ. Silybin inhibited mitochondria-associated endogenous apoptotic procedures and then improved the intestinal barrier function and morphology of PQ-challenged piglets. Moreover, silybin improved intestinal microbiota dysbiosis induced by the PQ challenge by enriching short-chain fatty-acid-producing bacteria, which augmented the production of acetate and propionate. Collectively, these findings indicated that dietary silybin supplementation linearly decreased feed conversion ratio and reduced diarrhea incidence in normal conditions, and effectively alleviated oxidative stress-induced mitochondrial dysfunction, intestinal damage, and microflora dysbiosis in weaned piglets.
引用
收藏
页数:20
相关论文
共 58 条
[1]   Gut microbiota-derived short chain fatty acids are potential mediators in gut inflammation [J].
Akhtar, Muhammad ;
Chen, Yan ;
Ma, Ziyu ;
Zhang, Xiaolong ;
Shi, Deshi ;
Khan, Jawaria A. ;
Liu, Huazhen .
ANIMAL NUTRITION, 2022, 8 :350-360
[2]   The Role of Intestinal Mucosal Barrier in Autoimmune Disease: A Potential Target [J].
An, Jia ;
Liu, Yuqing ;
Wang, Yiqi ;
Fan, Ru ;
Hu, Xiaorong ;
Zhang, Fen ;
Yang, Jinhua ;
Chen, Junwei .
FRONTIERS IN IMMUNOLOGY, 2022, 13
[3]   Mitochondria: from bioenergetics to the metabolic regulation of carcinogenesis [J].
Bellance, Nadege ;
Lestienne, Patrick ;
Rossignol, Rodrigue .
FRONTIERS IN BIOSCIENCE-LANDMARK, 2009, 14 :4015-4034
[4]   Silybin, a Major Bioactive Component of Milk Thistle (Silybum marianum L. Gaernt.)-Chemistry, Bioavailability, and Metabolism [J].
Bijak, Michal .
MOLECULES, 2017, 22 (11)
[5]  
Callahan BJ, 2016, NAT METHODS, V13, P581, DOI [10.1038/NMETH.3869, 10.1038/nmeth.3869]
[6]   Effect of paraquat on cytotoxicity involved in oxidative stress and inflammatory reaction: A review of mechanisms and ecological implications [J].
Chen, Jiaxin ;
Su, Yalin ;
Lin, Fei ;
Iqbal, Mujahid ;
Mehmood, Khalid ;
Zhang, Hui ;
Shi, Dayou .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2021, 224
[7]   Mitochondrial Complex I Inhibition in Dopaminergic Neurons Causes Altered Protein Profile and Protein Oxidation: Implications for Parkinson's disease [J].
Chithra, Yogeshachar ;
Dey, Gourav ;
Ghose, Vivek ;
Chandramohan, Vivek ;
Gowthami, Niya ;
Vasudev, V. ;
Bharath, M. M. Srinivas .
NEUROCHEMICAL RESEARCH, 2023, 48 (08) :2360-2389
[8]   Cell death: a review of the major forms of apoptosis, necrosis and autophagy [J].
D'Arcy, Mark S. .
CELL BIOLOGY INTERNATIONAL, 2019, 43 (06) :582-592
[9]   Multidrug Resistance Modulation Activity of Silybin Derivatives and Their Anti-Inflammatory Potential [J].
Dobiasova, Simona ;
Rehorova, Katerina ;
Kucerova, Denisa ;
Biedermann, David ;
Kanova, Kristyna ;
Petraskova, Lucie ;
Koucka, Kamila ;
Vaclavikova, Radka ;
Valentova, Katerina ;
Ruml, Tomas ;
Macek, Tomas ;
Kren, Vladimir ;
Viktorova, Jitka .
ANTIOXIDANTS, 2020, 9 (05)
[10]   Resveratrol and Montelukast Alleviate Paraquat-Induced Hepatic Injury in Mice: Modulation of Oxidative Stress, Inflammation, and Apoptosis [J].
El-Boghdady, Noha A. ;
Abdeltawab, Nourtan F. ;
Nooh, Mohammed M. .
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2017, 2017