Delayed host mortality and immune response upon infection with P. aeruginosa persister cells
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作者:
Hastings, Cody J.
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Binghamton Univ, Dept Biol Sci, Binghamton, NY 13902 USA
Binghamton Univ, Binghamton Biofilm Res Ctr, Binghamton, NY 13902 USABinghamton Univ, Dept Biol Sci, Binghamton, NY 13902 USA
Hastings, Cody J.
[1
,2
]
Keledjian, Maya V.
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机构:
Binghamton Univ, Dept Biol Sci, Binghamton, NY 13902 USA
Binghamton Univ, Binghamton Biofilm Res Ctr, Binghamton, NY 13902 USABinghamton Univ, Dept Biol Sci, Binghamton, NY 13902 USA
Keledjian, Maya V.
[1
,2
]
Musselman, Laura Palanker
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机构:
Binghamton Univ, Dept Biol Sci, Binghamton, NY 13902 USABinghamton Univ, Dept Biol Sci, Binghamton, NY 13902 USA
Musselman, Laura Palanker
[1
]
Marques, Claudia N. H.
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机构:
Binghamton Univ, Dept Biol Sci, Binghamton, NY 13902 USA
Binghamton Univ, Binghamton Biofilm Res Ctr, Binghamton, NY 13902 USABinghamton Univ, Dept Biol Sci, Binghamton, NY 13902 USA
Marques, Claudia N. H.
[1
,2
]
机构:
[1] Binghamton Univ, Dept Biol Sci, Binghamton, NY 13902 USA
[2] Binghamton Univ, Binghamton Biofilm Res Ctr, Binghamton, NY 13902 USA
Chronic infections are a heavy burden on healthcare systems worldwide. Persister cells are thought to be largely responsible for chronic infection due to their tolerance to antimicrobials and recalcitrance to innate immunity factors. Pseudomonas aeruginosa is a common and clinically relevant pathogen that contains stereotypical persister cells. Despite their importance in chronic infection, there have been limited efforts to study persister cell infections in vivo. Drosophila melanogaster has a well-described innate immune response similar to that of vertebrates and is a good candidate for the development of an in vivo model of infection for persister cells. Similar to what is observed in other bacterial strains, in this work we found that infection with P. aeruginosa persister cells resulted in a delayed mortality phenotype in Caenorhabditis elegans, Arabidopsis thaliana, and D. melanogaster compared to infection with regular cells. An in-depth characterization of infected D. melanogaster found that bacterial loads differed between persister and regular cells' infections during the early stages. Furthermore, hemocyte activation and antimicrobial peptide expression were delayed/reduced in persister infections over the same time course, indicating an initial suppression of, or inability to elicit, the fly immune response. Overall, our findings support the use of D. melanogaster as a model in which to study persister cells in vivo, where this bacterial subpopulation exhibits delayed virulence and an attenuated immune response.
机构:
Boston Univ, Dept Biomed Engn, Howard Hughes Med Inst, Boston, MA 02215 USA
Boston Univ, Ctr BioDynam, Boston, MA 02215 USABoston Univ, Dept Biomed Engn, Howard Hughes Med Inst, Boston, MA 02215 USA
Allison, Kyle R.
;
Brynildsen, Mark P.
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Princeton Univ, Dept Chem & Biol Engn, Princeton, NJ 08544 USABoston Univ, Dept Biomed Engn, Howard Hughes Med Inst, Boston, MA 02215 USA
Brynildsen, Mark P.
;
Collins, James J.
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机构:
Boston Univ, Dept Biomed Engn, Howard Hughes Med Inst, Boston, MA 02215 USA
Boston Univ, Ctr BioDynam, Boston, MA 02215 USA
Boston Univ, Sch Med, Boston, MA 02118 USA
Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02118 USABoston Univ, Dept Biomed Engn, Howard Hughes Med Inst, Boston, MA 02215 USA
机构:
Boston Univ, Howard Hughes Med Inst, Dept Biomed Engn, Ctr Biodynam, Boston, MA 02215 USABoston Univ, Howard Hughes Med Inst, Dept Biomed Engn, Ctr Biodynam, Boston, MA 02215 USA
Allison, Kyle R.
;
Brynildsen, Mark P.
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Boston Univ, Howard Hughes Med Inst, Dept Biomed Engn, Ctr Biodynam, Boston, MA 02215 USABoston Univ, Howard Hughes Med Inst, Dept Biomed Engn, Ctr Biodynam, Boston, MA 02215 USA
Brynildsen, Mark P.
;
Collins, James J.
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机构:
Boston Univ, Howard Hughes Med Inst, Dept Biomed Engn, Ctr Biodynam, Boston, MA 02215 USA
Boston Univ, Sch Med, Boston, MA 02118 USA
Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02118 USABoston Univ, Howard Hughes Med Inst, Dept Biomed Engn, Ctr Biodynam, Boston, MA 02215 USA
机构:
Univ Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil
SUNY Binghamton, Dept Biol Sci, Binghamton, NY USAUniv Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil
Almeida-Oliveira, Fernanda
;
Tuthill, Bryon F., II
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SUNY Binghamton, Dept Biol Sci, Binghamton, NY USAUniv Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil
Tuthill, Bryon F., II
;
Gondim, Katia C.
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Univ Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil
Inst Nacl Ciencia & Tecnol Entomol Mol, Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil
Gondim, Katia C.
;
Majerowicz, David
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机构:
Inst Nacl Ciencia & Tecnol Entomol Mol, Rio De Janeiro, Brazil
Univ Fed Rio de Janeiro, Fac Farm, Dept Biotecnol Farmaceut, Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil
Majerowicz, David
;
Musselman, Laura Palanker
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机构:
SUNY Binghamton, Dept Biol Sci, Binghamton, NY USAUniv Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil
机构:
Aligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, IndiaAligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, India
Azam, Mohd W.
;
Khan, Asad U.
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Aligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, IndiaAligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, India
机构:
Boston Univ, Dept Biomed Engn, Howard Hughes Med Inst, Boston, MA 02215 USA
Boston Univ, Ctr BioDynam, Boston, MA 02215 USABoston Univ, Dept Biomed Engn, Howard Hughes Med Inst, Boston, MA 02215 USA
Allison, Kyle R.
;
Brynildsen, Mark P.
论文数: 0引用数: 0
h-index: 0
机构:
Princeton Univ, Dept Chem & Biol Engn, Princeton, NJ 08544 USABoston Univ, Dept Biomed Engn, Howard Hughes Med Inst, Boston, MA 02215 USA
Brynildsen, Mark P.
;
Collins, James J.
论文数: 0引用数: 0
h-index: 0
机构:
Boston Univ, Dept Biomed Engn, Howard Hughes Med Inst, Boston, MA 02215 USA
Boston Univ, Ctr BioDynam, Boston, MA 02215 USA
Boston Univ, Sch Med, Boston, MA 02118 USA
Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02118 USABoston Univ, Dept Biomed Engn, Howard Hughes Med Inst, Boston, MA 02215 USA
机构:
Boston Univ, Howard Hughes Med Inst, Dept Biomed Engn, Ctr Biodynam, Boston, MA 02215 USABoston Univ, Howard Hughes Med Inst, Dept Biomed Engn, Ctr Biodynam, Boston, MA 02215 USA
Allison, Kyle R.
;
Brynildsen, Mark P.
论文数: 0引用数: 0
h-index: 0
机构:
Boston Univ, Howard Hughes Med Inst, Dept Biomed Engn, Ctr Biodynam, Boston, MA 02215 USABoston Univ, Howard Hughes Med Inst, Dept Biomed Engn, Ctr Biodynam, Boston, MA 02215 USA
Brynildsen, Mark P.
;
Collins, James J.
论文数: 0引用数: 0
h-index: 0
机构:
Boston Univ, Howard Hughes Med Inst, Dept Biomed Engn, Ctr Biodynam, Boston, MA 02215 USA
Boston Univ, Sch Med, Boston, MA 02118 USA
Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02118 USABoston Univ, Howard Hughes Med Inst, Dept Biomed Engn, Ctr Biodynam, Boston, MA 02215 USA
机构:
Univ Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil
SUNY Binghamton, Dept Biol Sci, Binghamton, NY USAUniv Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil
Almeida-Oliveira, Fernanda
;
Tuthill, Bryon F., II
论文数: 0引用数: 0
h-index: 0
机构:
SUNY Binghamton, Dept Biol Sci, Binghamton, NY USAUniv Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil
Tuthill, Bryon F., II
;
Gondim, Katia C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil
Inst Nacl Ciencia & Tecnol Entomol Mol, Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil
Gondim, Katia C.
;
Majerowicz, David
论文数: 0引用数: 0
h-index: 0
机构:
Inst Nacl Ciencia & Tecnol Entomol Mol, Rio De Janeiro, Brazil
Univ Fed Rio de Janeiro, Fac Farm, Dept Biotecnol Farmaceut, Rio De Janeiro, BrazilUniv Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil
Majerowicz, David
;
Musselman, Laura Palanker
论文数: 0引用数: 0
h-index: 0
机构:
SUNY Binghamton, Dept Biol Sci, Binghamton, NY USAUniv Fed Rio de Janeiro, Inst Bioquim Med Leopoldo de Meis, Rio De Janeiro, Brazil
机构:
Aligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, IndiaAligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, India
Azam, Mohd W.
;
Khan, Asad U.
论文数: 0引用数: 0
h-index: 0
机构:
Aligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, IndiaAligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, India