Structural characterization and anti-inflammatory effects of an arabinan isolated from Rehmannia glutinosa Libosch.

被引：30

作者：

Zhang, Han ^{[1
,4
]}

Yue, Yimin ^{[1
,2
]}

Zhang, Qian ^{[3
]}

Liang, Lanyuan ^{[1
,2
]}

Li, Chuanqiu ^{[1
,2
]}

Chen, Ying ^{[1
,2
]}

Li, Wei ^{[1
,2
]}

Peng, Mingming ^{[1
,2
]}

Yang, Mengru ^{[1
,2
]}

Zhao, Mantong ^{[1
,2
]}

Cao, Xia ^{[1
,2
]}

Zhong, Luyang ^{[1
,2
]}

Du, Jieyong ^{[1
,2
]}

Wang, Yi ^{[1
,2
]}

Zhou, Xi ^{[5
,6
,7
]}

Shu, Zunpeng ^{[1
,2
,8
]}

机构：

[1] Guangdong Pharmaceut Univ, Guangdong Prov Key Lab Adv Drug Delivery, Guangzhou 510006, Peoples R China

[2] Guangdong Pharmaceut Univ, Sch Chinese Mat Med, Guangzhou 510006, Peoples R China

[3] Guangdong Pharmaceut Univ, Sch Pharm, Guangzhou 510006, Peoples R China

[4] Jiamusi Univ, Sch Pharm, Jiamusi 154007, Peoples R China

[5] China Natl Analyt Ctr, Guangdong Prov Key Lab Chem Measurement & Emergenc, Guangzhou 510006, Peoples R China

[6] China Natl Analyt Ctr, Guangdong Prov Engn Res Ctr Qual & Safety Tradit C, Guangzhou 510006, Peoples R China

[7] Guangdong Acad Sci, China Natl Analyt Ctr, Inst Anal, Guangzhou 510006, Peoples R China

[8] Guangdong Pharmaceut Univ, 280 Guangzhou Higher Educ Mega Ctr, Guangzhou 510006, Guangdong Provi, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2023年 / 303卷

关键词：

Rehmannia glutinosa Libosch; Arabinan; Anti-inflammatory effect; ROS-NF-KB pathway; ACIDIC POLYSACCHARIDE; ELUCIDATION; SPECTROSCOPY; GLUCAN;

D O I：

10.1016/j.carbpol.2022.120441

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Considering that natural polysaccharides are potential anti-inflammatory agents, in this study, an arabinan (RGP70-2) was isolated and purified from Rehmannia glutinosa Libosch. (R. glutinosa) and its structure was characterized. RGP70-2 was a homogeneous polysaccharide with a molecular weight of 6.7 kDa, with the main backbone comprising-*5)-alpha-L-Araf-(1-*,-*3)-alpha-L-Araf-(1-*,-*2,3,5)-alpha-L-Araf-(1-*, and-*2,5)-alpha-L-Araf-(1-* linkages and the side chain comprising an alpha-L-Araf-(1-* linkage. In vivo experiments showed that RGP70-2 inhibited ROS production and downregulated the expression of pro-inflammatory cytokines (TNF-alpha, IL-1 beta, and IL-6). In vitro experiments showed that RGP70-2 decreased levels of pro-inflammatory cytokines, inhibited ROS production, and attenuated NF-KB-p65 translocation from the cytoplasm to the nucleus. Our results showed that RGP70-2 may delay inflammation by regulating the ROS-NF-KB pathway. Thus, RGP70-2 has potential appli-cations as an anti-inflammatory agent in the biopharmaceutical industry.

引用

页数：13

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