Tracing the Anti-cancer Mechanism of Pleurotus osteratus by the Integrative Approach of Network Pharmacology and Experimental Studies

被引：11

作者：

Shreya, Singh ^{[1
]}

Kumar, Dulla Naveen ^{[2
]}

Mohapatra, Debadatta ^{[1
]}

Jaiswal, Shivani ^{[3
]}

Naik, Gaurav Gopal ^{[1
]}

Guru, Santosh Kumar ^{[4
]}

Agarwal, Ashish Kumar ^{[2
]}

Ayyannan, Senthil Raja ^{[3
]}

Sahu, Alakh N. ^{[1
]}

机构：

[1] Indian Inst Technol BHU, Dept Pharmaceut Engn & Technol, Phytomed Res Lab, Varanasi 221005, Uttar Pradesh, India

[2] Indian Inst Technol BHU, Dept Pharmaceut Engn & Technol, Nanomed Res Lab, Varanasi 221005, Uttar Pradesh, India

[3] Indian Inst Technol BHU, Dept Pharmaceut Engn & Technol, Pharmaceut Chem Res Lab 2, Varanasi 221005, Uttar Pradesh, India

[4] NIPER Hyderabad, Dept Biol Sci, Dept Pharmaceut, Hyderabad 500037, Telangana, India

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2023年 / 195卷 / 01期

关键词：

Anti-cancer; EAC bearing Swiss albino mice; Network pharmacology; PI3K-AKT-mTOR; Pleurotus osteratus; TAMOXIFEN RESISTANCE; RECEPTOR;

D O I：

10.1007/s12010-022-04111-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study identified the probable mechanism behind the anti-cancer activity of the hexane fraction of Pleurotus osteratus (HFPO) using network pharmacology and experimental validation. HFPO myco-metabolites targets and targets related to the cancer were mined from the online web server, and overlapping targets were screened. Out of the 74 overlapping targets, 33 targets were identified in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway of cancer. Furthermore, the main active myco-metabolites and hub targets were identified by network analysis of the compound-targets network and protein-protein interaction (PPI), respectively. Molecular docking results showed good binding affinity of the hub targets with their respective myco-metabolites. HFPO induced in-vitro anti-cancer activity by affecting the PI3K-AKT-mTOR pathway, besides time-dependent cell cytotoxicity and apoptotic cell body formation. Additionally, tumor volume reduction was observed in HFPO-treated Ehrlich ascites carcinoma (EAC) bearing Swiss albino mice. Overall, HFPO induces anti-cancer potential by modulating the PI3K-AKT-mTOR signaling pathway.

引用

页码：152 / 171

页数：20

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