Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root

被引：77

作者：

Nolan, Trevor M. ^{[1
]}

Vukasinovic, Nemanja ^{[2
,3
]}

Hsu, Che-Wei ^{[1
,4
,5
]}

Zhang, Jingyuan ^{[1
]}

Vanhoutte, Isabelle ^{[2
,3
]}

Shahan, Rachel ^{[1
,6
]}

Taylor, Isaiah W. ^{[1
]}

Greenstreet, Laura ^{[7
]}

Heitz, Matthieu ^{[7
]}

Afanassiev, Anton ^{[7
]}

Wang, Ping ^{[8
]}

Szekely, Pablo ^{[1
,6
]}

Brosnan, Aiden ^{[1
]}

Yin, Yanhai ^{[8
]}

Schiebinger, Geoffrey ^{[7
]}

Ohler, Uwe ^{[4
,8
,9
]}

Russinova, Eugenia ^{[2
,3
]}

Benfey, Philip N. ^{[1
,6
]}

机构：

[1] Duke Univ, Dept Biol, Durham, NC 27708 USA

[2] Univ Ghent, Dept Plant Biotechnol & Bioinformat, Ghent, Belgium

[3] VIB, Ctr Plant Syst Biol, Ghent, Belgium

[4] Humboldt Univ, Dept Biol, Berlin, Germany

[5] Max Delbruck Ctr Mol Med, Berlin Inst Med Syst Biol, Berlin, Germany

[6] Duke Univ, Howard Hughes Med Inst, Durham, NC 27708 USA

[7] Univ British Columbia, Dept Math, Vancouver, BC, Canada

[8] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA USA

[9] Humboldt Univ, Dept Comp Sci, Berlin, Germany

来源：

SCIENCE | 2023年 / 379卷 / 6639期

基金：

加拿大自然科学与工程研究理事会; 美国食品与农业研究所; 美国国家卫生研究院; 美国国家科学基金会;

关键词：

PLANT-GROWTH; TRANSCRIPTION FACTORS; EXTRACELLULAR DOMAIN; CLASS-I; EXPRESSION; MERISTEM; KINASE; BIOSYNTHESIS; DIFFERENTIATION; PERCEPTION;

D O I：

10.1126/science.adf4721

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Brassinosteroids are plant steroid hormones that regulate diverse processes, such as cell division and cell elongation, through gene regulatory networks that vary in space and time. By using time series single-cell RNA sequencing to profile brassinosteroid-responsive gene expression specific to different cell types and developmental stages of the Arabidopsis root, we identified the elongating cortex as a site where brassinosteroids trigger a shift from proliferation to elongation associated with increased expression of cell wall-related genes. Our analysis revealed HOMEOBOX FROM ARABIDOPSIS THALIANA 7 (HAT7) and GT-2-LIKE 1 (GTL1) as brassinosteroid-responsive transcription factors that regulate cortex cell elongation. These results establish the cortex as a site of brassinosteroid-mediated growth and unveil a brassinosteroid signaling network regulating the transition from proliferation to elongation, which illuminates aspects of spatiotemporal hormone responses.

引用

页码：1314 / +

页数：13

共 86 条

[1] Auxin requirements for a meristematic state in roots depend on a dual brassinosteroid function [J].