Novel vancomycin free base-Sterosomes for combating diseases caused by Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus infections (S. Aureus and MRSA)

被引：0

作者：

Nwabuife, Joshua C. ^{[1
]}

Hassan, Daniel ^{[1
]}

Pant, Amit Madhaorao ^{[1
]}

Devnarain, Nikita ^{[1
]}

Gafar, Mohammed Ali ^{[1
]}

Osman, Nawras ^{[1
]}

Rambharose, Sanjeev ^{[2
]}

Govender, Thirumala ^{[1
]}

机构：

[1] Univ KwaZulu Natal, Coll Hlth Sci, Discipline Pharmaceut Sci, ZA-4000 Durban, South Africa

[2] Stellenbosch Univ, Fac Sci, Dept Physiol Sci, Private Bag X1 Matieland, ZA-7602 Stellenbosch, South Africa

来源：

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY | 2023年 / 79卷

基金：

新加坡国家研究基金会; 英国医学研究理事会;

关键词：

Sterosomes; Novel delivery systems; Antimicrobial resistance; DRUG-DELIVERY SYSTEMS; IN-VITRO; NANOPARTICLES; ACID; NANOTECHNOLOGY; NANOSYSTEM; NANOSPHERES; PATHOGENS; EFFICACY; POLYMER;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A clarion call to save antimicrobials is the need for the evolution of novel delivery systems to combat resistance posed by Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (S. aureus and MRSA) to conventional forms. Herein, we present a novel vancomycin free base - sterosomes (VCM-FB - Sterosomes) for vancomycin free base (VCM-FB) delivery against S. aureus and MRSA. VCM-FB - Sterosomes were prepared using the thin-film hydration method and were characterised physiochemically, in silico, in vitro and in vivo. In vitro cytotoxicity on MCF-7 and HEK-293 cell lines were evaluated, with results revealing cell viability above 70% after exposure to VCM-FB - Sterosomes, indicating biosafety. VCM-FB - Sterosomes had a hydrodynamic diameter, polydispersity index and surface-charge of 114.14 +/- 0.59 nm, 0.210 +/- 0.02 and +36.3 +/- 5.42 mV, respectively, with entrapment efficiency, drug loading and VCM-FB release after 48 h of 79.61 +/- 0.59%, 90.91% w/w and 54.95 +/- 9.75% respectively. In silico studies showed a self-assembly in five nano seconds, which was stable thereafter. VCM-FB - Sterosomes were seen to be stable over 90 days and were also non-hemolytic. VCMFB - Sterosomes showed a 2-fold superior minimum inhibition efficiency (MIC) against S. aureus and MRSA, relative to bare VCM-FB, with MICs of 1.95 mu g/mL and 0.98 mu g/mL for VCM-FB and VCM-FB - Sterosomes (S. aureus), and 7.81 mu g/mL and 3.91 mu g/mL for VCM-FB and VCM-FB - Sterosomes (MRSA), respectively. A faster bacterial killing time was also recorded for VCM-FB - Sterosomes compared to bare VCM-FB. Greater MRSA membrane damage was indicated by an increase and decrease in electrical conductivity, and protein/DNA concentration, respectively. VCM-FB - Sterosomes showed superior MRSA biofilm reduction (27.22%) compared to VCM-FB (2.53%). In vivo BALB/c mice-infected skin model showed that VCM-FB - Sterosomes had significant MRSA eradication (27-fold) compared to bare VCM-FB (4-fold). These results amplify the superiority of VCM-FB - Sterosomes for S. aureus and MRSA infection treatment compared to conventional forms.

引用

页数：13

共 77 条

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