The study focused on examining transportation operations and environmental sustainability in manufacturing firms in Lagos State, Nigeria. The focal objective of the study is to analyse transportation optimality effect on environmental sustainability and its utilisation in order to minimise carbon emissions due to transportation in production activities and distribution. Twelve manufacturing firms were utilised for the study and the firms were selected based on the volume of distribution. Multi-method such as partial least square (PLS) and Vogel's estimation model was utilised to determine the resultant effect of transportation on environmental sustainability and its corresponding optimal routes. The coefficient value of 0.601 based on the statistical output shows a considerable effect of transportation on environmental sustainability. This shows a significant contribution to environmental degradation due to the sporadic growth in reverse order, distribution and allocation. The study also discovered that transportation optimality is an impetus to environmental sustainability due to optimal distribution and routes.