Synthetic developmental biology: New tools to deconstruct and rebuild developmental systems

被引:14
作者
McNamara, Harold M. [1 ,2 ,3 ]
Ramm, Beatrice [2 ,3 ]
Toettcher, Jared E. [3 ,4 ]
机构
[1] Princeton Univ, Lewis Sigler Inst, Princeton, NJ 08544 USA
[2] Princeton Univ, Dept Phys, Princeton, NJ 08544 USA
[3] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA
[4] Lewis Thomas Lab Room 140, Washington Rd, Princeton, NJ 08544 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
Synthetic biology; Gene circuits; Optogenetics; Development; GENE-EXPRESSION SYSTEM; SPATIOTEMPORAL CONTROL; OPTOGENETIC CONTROL; CELL FATE; IN-VIVO; CIRCUITS; ACTIVATION; FEEDBACK; DYNAMICS; NETWORK;
D O I
10.1016/j.semcdb.2022.04.013
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Technological advances have driven many recent advances in developmental biology. Light sheet imaging can reveal single-cell dynamics in living three-dimensional tissues, whereas single-cell genomic methods open the door to a complete catalogue of cell types and gene expression states. An equally powerful but complementary set of approaches are also becoming available to define development processes from the bottom up. These synthetic approaches aim to reconstruct the minimal developmental patterns, signaling processes, and gene networks that produce the basic set of developmental operations: spatial polarization, morphogen interpretation, tissue movement, and cellular memory. In this review we discuss recent approaches at the intersection of synthetic biology and development, including synthetic circuits to deliver and record signaling stimuli and synthetic reconstitution of pattern formation on multicellular scales.
引用
收藏
页码:33 / 42
页数:10
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