Direct observation of cytoskeleton-dependent trafficking of miRNA visualized by the introduction of pre-miRNA

被引：4

作者：

Ishikawa, Toshinari ^{[1
]}

Sugawara, Ko ^{[1
]}

Zhang, Junwei ^{[1
]}

Funatsu, Takashi ^{[1
]}

Okabe, Kohki ^{[1
,2
]}

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, 7-3-1 Hongo,Bunkyo Ku, Tokyo 1130033, Japan

[2] JST, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 3320012, Japan

来源：

ISCIENCE | 2024年 / 27卷 / 02期

基金：

日本科学技术振兴机构;

关键词：

MESSENGER-RNAS; ENDOPLASMIC-RETICULUM; MICRORNA BIOGENESIS; TRANSLATION; PROTEIN; LOCALIZATION; TURNOVER;

D O I：

10.1016/j.isci.2024.108811

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

MicroRNA (miRNA) plays physiologically and pathologically important roles in post -transcriptional regulation. Although miRNA has been suggested to dynamically interact with cellular organelles, the dynamicity of intracellular miRNA behavior has remained unclear. Here, by introducing fluorescently labeled pre-miRNA into living cells, we improved the miRNA visualization method using exogenous miRNA precursors. Through the combination of our miRNA visualization method and single -molecule sensitive fluorescence microscopy, we quantitatively analyzed the process of miRNA maturation. Furthermore, singleparticle tracking of fluorescent miRNA in cells revealed the directed movements of miRNA on cytoskeletal components (i.e., microtubules and actin filaments). Our results also suggest that cytoskeleton-dependent miRNA trafficking is associated with the interaction of miRNAs with the nucleus and the endoplasmic reticulum/Golgi apparatus. Our method should facilitate the elucidation of the mechanism and physiological significance of the subcellular localization and organelle interaction of miRNA.

引用

页数：15

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