Isolation, identification, and pathogenicity of porcine epidemic diarrhea virus

被引:13
作者
Sun, Yingshuo [1 ,2 ]
Gong, Ting [1 ,3 ]
Wu, Dongdong [1 ,2 ]
Feng, Yongzhi [1 ,3 ]
Gao, Qi [1 ,3 ]
Xing, Jiabao [1 ,2 ]
Zheng, Xiaoyu [1 ,3 ]
Song, Zebu [1 ,3 ]
Liu, Xing [1 ]
Chen, Xiongnan [1 ,3 ]
Sun, Yankuo [1 ,3 ]
Zhang, Guihong [1 ,2 ,3 ]
Gong, Lang [1 ,2 ,3 ]
机构
[1] South China Agr Univ, Coll Vet Med, Guangdong Prov Key Lab Zoonosis Prevent & Control, Guangzhou, Peoples R China
[2] Minist Agr & Rural Affairs, Key Lab Anim Vaccine Dev, Guangzhou, Peoples R China
[3] Guangdong Lab Lingnan Modern Agr Sci & Technol, Zhaoqing Branch Ctr, Zhaoqing, Peoples R China
关键词
porcine epidemic diarrhea virus; mutation; nsp1; identification; pathogenicity; INFECTION;
D O I
10.3389/fmicb.2023.1273589
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Porcine epidemic diarrhea (PED) is an enterophilic infectious disease caused by the porcine epidemic diarrhea virus (PEDV), which can lead to dehydration-like diarrhea in piglets with a mortality rate of up to 100%, causing huge economic losses to the global pig industry. In this study, we isolated two PEDV strains, FS202201 and JY202201, from diarrheal samples collected from two new PED outbreak farms in 2022. We performed phylogenetic analysis of the S gene and whole gene sequence. The effects of the different mutations on viral pathogenicity were investigated using piglet challenge experiments. The results showed that both strains belong to the G2c subtype, a widely prevalent virulent strain. Compared with FS202201, JY202201 harbored substitution and deletion mutations in nsp1. Both FS202201 and JY202201 infected piglets showed severe diarrhea and significant intestinal tissue lesions at an infection dose of 104 TCID50/mL, with a mortality rate of 50%; however, JY202201 required an additional day to reach mortality stabilization. An infection dose of 103 TCID50/mL reduced diarrhea and intestinal tissue lesions in piglets, with mortality rates of the two strains at 16.7% and 0%, respectively. In addition, PEDV was detected in the heart, liver, spleen, lungs, kidneys, mesenteric lymph nodes, stomach, large intestine, duodenum, jejunum, and ileum, with the highest levels in the intestinal tissues. In conclusion, this study enriches the epidemiology of PEDV and provides a theoretical basis for the study of its pathogenic mechanism and prevention through virus isolation, identification, and pathogenicity research on newly identified PED in the main transmission hub area of PEDV in China (Guangdong).
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页数:10
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