Enrichment and Selection of Particles through Parallel Induced-Charge Electro-osmotic Streaming for Detection of Low-Abundance Nanoparticles and Targeted Microalgae

被引:10
作者
Chen, Xiaoming [1 ,2 ]
Liu, Shun [1 ,2 ]
Hu, Xu-guang [1 ,2 ]
Liu, Tengteng [1 ,2 ]
Shen, Mo [1 ,2 ]
Peng, Yun [1 ,2 ]
Hu, Sheng [1 ,2 ]
Zhao, Yong [1 ,2 ]
机构
[1] Northeastern Univ Qinhuangdao, Sch Control Engn, Qinhuangdao 066004, Peoples R China
[2] Hebei Key Lab Micronano Precis Opt Sensing & Measu, Qinhuangdao 066004, Peoples R China
基金
中国国家自然科学基金;
关键词
BIPOLAR ELECTRODE; WHOLE-BLOOD; SEPARATION; DIELECTROPHORESIS; CAPTURE; DESIGN; CELLS; CHIP;
D O I
10.1021/acs.analchem.3c01729
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Manipulation of micro- and nanoscale objects is an essentialprocedurein many detection and sensing applications, including disease diagnosisand environmental monitoring. Induced-charge electro-osmotic (ICEO)vortices present excellent advantages in the enrichment and selectionof micro/nanoscale particles for downstream detection due to gentleconditions and contactless operation, but the application of thismethod is currently constrained by the throughput. Double-layer chargingat the ends of bipolar electrodes can maintain a continuous flow ofelectric current in the fluidically isolated channels, which providesa feasible method to manipulate particles using parallel ICEO vortices,promoting throughput of particle manipulation without compromisingefficiency and overcoming the complicated ohmic contact of electrodes.Encouraged by these, we put forward a novel method with parallel ICEOvortices to manipulate micro/nanoscale samples for downstream detection.First, we study the extension regulation of the low-frequency electricfield and mediating effect of the open BPEs on the extended electricfield and characterize electric equilibrium states of microparticlesand their voltage dependence. Afterward, we leverage this method toenrich nanoparticles for detection of low-abundance nanoparticleswith about 20- and 40-fold fluorescence intensities by integratingwith a simple fiber-optic sensor. Furthermore, this technique is engineeredfor the selection of targeted microalgae to continuously detect theirproliferation behaviors by combining with a homemade electrical impedancespectroscopy device. This method can reinforce the throughput of ICEOvortices and enables it to integrate with simple and economical sensorsto accomplish disease diagnosis and environmental monitoring.
引用
收藏
页码:11714 / 11722
页数:9
相关论文
共 39 条
[1]   Rotational manipulation of single cells and organisms using acoustic waves [J].
Ahmed, Daniel ;
Ozcelik, Adem ;
Bojanala, Nagagireesh ;
Nama, Nitesh ;
Upadhyay, Awani ;
Chen, Yuchao ;
Hanna-Rose, Wendy ;
Huang, Tony Jun .
NATURE COMMUNICATIONS, 2016, 7
[2]   Negative Dielectrophoretic Capture and Repulsion of Single Cells at a Bipolar Electrode: The Impact of Faradaic Ion Enrichment and Depletion [J].
Anand, Robbyn K. ;
Johnson, Eleanor S. ;
Chiu, Daniel T. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (02) :776-783
[3]   Real-time label-free monitoring of adipose-derived stem cell differentiation with electric cell-substrate impedance sensing [J].
Bagnaninchi, Pierre O. ;
Drummond, Nicola .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (16) :6462-6467
[4]   Induced-charge electrokinetic phenomena: Theory and microfluidic applications [J].
Bazant, MZ ;
Squires, TM .
PHYSICAL REVIEW LETTERS, 2004, 92 (06) :661011-661014
[5]   Characterization of Particle Movement and High-Resolution Separation of Microalgal Cells via Induced-Charge Electroosmotic Advective Spiral Flow [J].
Chen, Xiaoming ;
Ren, Yukun ;
Jiang, Tianyi ;
Hou, Likai ;
Jiang, Hongyuan .
ANALYTICAL CHEMISTRY, 2021, 93 (03) :1667-1676
[6]   Induced charge electro-osmotic particle separation [J].
Chen, Xiaoming ;
Ren, Yukun ;
Hou, Likai ;
Feng, Xiangsong ;
Jiang, Tianyi ;
Jiang, Hongyuan .
NANOSCALE, 2019, 11 (13) :6410-6421
[7]   A Simplified Microfluidic Device for Particle Separation with Two Consecutive Steps: Induced Charge Electro-osmotic Prefocusing and Dielectrophoretic Separation [J].
Chen, Xiaoming ;
Ren, Yukun ;
Liu, Weiyu ;
Feng, Xiangsong ;
Jia, Yankai ;
Tao, Ye ;
Jiang, Hongyuan .
ANALYTICAL CHEMISTRY, 2017, 89 (17) :9583-9592
[8]   A Large-Scale, Wireless Electrochemical Bipolar Electrode Microarray [J].
Chow, Kwok-Fan ;
Mavre, Francois ;
Crooks, John A. ;
Chang, Byoung-Yong ;
Crooks, Richard M. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (24) :8364-+
[9]   Recent advancement in induced-charge electrokinetic phenomena and their micro- and nano-fluidic applications [J].
Feng, Huicheng ;
Chang, Honglong ;
Zhong, Xin ;
Wong, Teck Neng .
ADVANCES IN COLLOID AND INTERFACE SCIENCE, 2020, 280
[10]   Model based design of a microfluidic mixer driven by induced charge electroosmosis [J].
Harnett, Cindy K. ;
Templeton, Jeremy ;
Dunphy-Guzman, Katherine A. ;
Senousy, Yehya M. ;
Kanouff, Michael P. .
LAB ON A CHIP, 2008, 8 (04) :565-572