High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development

被引：49

作者：

Bevilacqua, Carlo ^{[1
,2
]}

Gomez, Juan Manuel ^{[3
]}

Fiuza, Ulla-Maj ^{[1
,9
]}

Chan, Chii Jou ^{[1
,10
,11
]}

Wang, Ling ^{[1
]}

Hambura, Sebastian ^{[1
]}

Eguren, Manuel ^{[1
]}

Ellenberg, Jan ^{[1
]}

Diz-Munoz, Alba ^{[1
]}

Leptin, Maria ^{[3
]}

Prevedel, Robert ^{[1
,4
,5
,6
,7
,8
]}

机构：

[1] European Mol Biol Lab, Cell Biol & Biophys Unit, Heidelberg, Germany

[2] Heidelberg Univ, Fac Biosci, Heidelberg, Germany

[3] European Mol Biol Lab, Directors Res Unit, Heidelberg, Germany

[4] European Mol Biol Lab, Dev Biol Unit, Heidelberg, Germany

[5] European Mol Biol Lab, Epigenet & Neurobiol Unit, Monterotondo, Italy

[6] European Mol Biol Lab, Mol Med Partnership Unit MMPU, Heidelberg, Germany

[7] German Ctr Lung Res DZL, Heidelberg, Germany

[8] Heidelberg Univ, Interdisciplinary Ctr Neurosci, Heidelberg, Germany

[9] Univ Pompeu Fabra, Syst Bioengn, MELIS, Barcelona, Spain

[10] Natl Univ Singapore, Mechanobiol Inst, Singapore, Singapore

[11] Natl Univ Singapore, Dept Biol Sci, Singapore, Singapore

来源：

NATURE METHODS | 2023年 / 20卷 / 05期

基金：

欧洲研究理事会;

关键词：

DROSOPHILA GASTRULATION; CONFOCAL BRILLOUIN;

D O I：

10.1038/s41592-023-01822-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Brillouin microscopy can assess mechanical properties of biological samples in a three-dimensional (3D), all-optical and hence non-contact fashion, but its weak signals often lead to long imaging times and require an illumination dosage harmful for living organisms. Here, we present a high-resolution line-scanning Brillouin microscope for multiplexed and hence fast 3D imaging of dynamic biological processes with low phototoxicity. The improved background suppression and resolution, in combination with fluorescence light-sheet imaging, enables the visualization of the mechanical properties of cells and tissues over space and time in living organism models such as fruit flies, ascidians and mouse embryos. Line-scan Brillouin microscopy enables fast 3D imaging of mechanical properties with low phototoxicity, as shown for Drosophila and mouse embryos, as well as ascidians.

引用

页码：755 / +

页数：25

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