The Effect of Protease Enzyme and Replacement Soybean Meal by Cotton Seed Meal on Protein Efficiency and Performance in Broiler Chickens

This study was conducted to investigate the effect of replacing soy bean meal (SBM) by cottonseed meal (CSM) with or without of protease enzymes, on performance, in-vitro protein digestibility, carcass charac-teristics, gastrointestinal morphology and hematological parameters in broilers. A total 450 one-day-old mixed sex broiler chickens Ross 308 were managed in 6 treatments, 5 replicates and 15 chickens in each in a 2 x 3 factorial arrangement with completely randomized design (CRD). Treatments consist of: treatment 1 (0% CSM,-protease), treatment 2 (0% CSM, +protease), treatment 3 (20% CSM,-protease), treatment 4 (20% CSM, +protease), treatment 5 (30% CSM,-protease), treatment 6 (30% CSM, +protease). The results have shown that there were no significant affected on feed intake, body weight gain and feed conversion ratio by various treatments in starter period (P>0.05). Digestive organs and body weight were not affected by treatments at 27 days of age. Breasts weight was significantly increased by 20% replacement SBM with CSM (P<0.05). Protein digestibility was increased significantly by protease enzymes and in vitro method. Serum cholesterol levels reduced by enhanced levels of replacement CSM (P<0.05). There was concluded that no adverse effects were found by replacing 30% SBM with CSM on performance status, as well as improved in vitro protein digestibility.