Protein cohabitation: long-term immunoglobulin G storage at room temperature

被引：0

作者：

Bharmoria, Pankaj ^{[1
,3
,4
]}

Ooi, Saik Ann ^{[1
]}

Cellini, Andrea ^{[1
]}

Tietze, Daniel ^{[1
]}

Maj, Michal ^{[2
]}

Moth-Poulsen, Kasper ^{[3
,4
,5
,6
]}

Tietze, Alesia A. ^{[1
]}

机构：

[1] Univ Gothenburg, Dept Chem & Mol Biol, Kemigarden 10, S-41296 Gothenburg, Sweden

[2] Angstrom Lab, Dept Chem, Phys Chem, Lagerhyddsvagen 1, S-75120 Uppsala, Sweden

[3] Chalmers Univ Technol, Dept Chem & Chem Engn, Kemivagen 4, S-41296 Gothenburg, Sweden

[4] ICMAB CSIC, Inst Mat Sci Barcelona, Barcelona 08193, Spain

[5] ICREA, Catalan Inst Res & Adv Studies, Pg Lluis Co 23, Barcelona, Spain

[6] Univ Politecn Cataluna, EEBE, Dept Chem Engn, Eduard Maristany 10-14, Barcelona 08019, Spain

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2023年 / 11卷 / 24期

基金：

瑞典研究理事会;

关键词：

STABILITY;

D O I：

10.1039/d3tb00161j

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Long-term functional storage of therapeutic proteins at room temperature has been an eternal challenge. Inspired by the cellular cooperativity of proteins, we have taken a step forward to address this challenge by cohabitating Immunoglobulin G (IgG1) with a food protein gelatin in the solid-state at room temperature. Interestingly, IgG1 remained functionally active for a record 14 months revealed from the western-blot assay. Further quantification by HP-LC analysis showed 100% structural integrity of IgG1 with no degradation in the gelatin matrix during this period. The developed formulation has a direct application in oral medical nutrition therapy to cure gastrointestinal microbial infections. Also the strategy provides a robust energy economic alternative to the protein engineering methods for long-term functional storage of therapeutic proteins at room temperature.

引用

页码：5400 / 5405

页数：6

共 64 条

[1] Peptide Dendrons as Thermal-Stability Amplifiers for Immunoglobulin G1 Monoclonal Antibody Biotherapeutics [J].