Protocol for simultaneous patch-clamp recording from tanycytes and neurons in living mouse brain slices

被引：2

作者：

Lhomme, Tori ^{[1
]}

Prevot, Vincent ^{[1
]}

机构：

[1] Univ Lille, CHU Lille, Lab Dev & Plast Neuroendocrine Brain, Inserm,Lille Neurosci & Cognit,UMRS 1172, F-59000 Lille, France

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 04期

基金：

欧洲研究理事会;

关键词：

Metabolism; Neuroscience;

D O I：

10.1016/j.xpro.2023.102571

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here, we present a protocol for tanycyte-neuron paired whole-cell patch-clamp recording in living mouse brain slices. We describe steps for mice generation, solution preparation, and dissection. We then detail realization of slices and patch-clamp recordings. While we use, as an example, tanycytes of the arcuate nucleus of the hypothalamus and pro-opiomelanocortin neurons, this protocol can be adapted to study metabolic coupling between tanycytes and any neuronal population.For complete details on the use and execution of this protocol, please refer to Lhomme et al. (2021).1

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页数：23

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