Protocol for high-throughput single-cell patterning using a reusable ultrathin metal microstencil

被引：0

作者：

Tian, Qingqing ^{[1
]}

Xing, Kunming ^{[2
]}

Liu, Yongshu ^{[1
]}

Wang, Qian ^{[1
]}

Sun, Haonan ^{[1
]}

Sun, Ying-Nan ^{[1
]}

Zhang, Shusheng ^{[1
]}

机构：

[1] Linyi Univ, Sch Med, Shandong Prov Key Lab Detect Technol Tumor Makers, Linyi 276005, Shandong, Peoples R China

[2] Linyi Peoples Hosp, Linyi 276100, Shandong, Peoples R China

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Biotechnology and bioengineering; Cell Biology; Cell culture; Cell isolation; High Throughput Screening; Single Cell;

D O I：

10.1016/j.xpro.2023.102115

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Exploiting convenient strategies for single-cell preparation while maintaining a high throughput remains challenging. This protocol describes a simple workflow for high-throughput single-cell patterning using a reusable ultrathin metal micro -stencil (UTmS). We describe UTmS-chip design, fabrication, and quality charac-terization. We then detail the preparation of flat substrates and chip assembly for single-cell patterning, followed by culturing of cells on a chip. Finally, we describe the evaluation of single-cell patterning and the downstream applica-tions for studying single-cell calcium release and apoptosis.For complete details on the use and execution of this protocol, please refer to Song et al. (2021).1

引用

页数：25

共 14 条

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