Precise digital bacteria enumeration and antibiotic susceptibility testing via a portable vibrating capillary-based droplet platform

被引：8

作者：

Ding, Chizhu ^{[1
]}

Liu, Yue ^{[1
]}

Guo, Yan ^{[1
]}

Guo, Xiaoting ^{[1
]}

Kang, Qinshu ^{[1
]}

Yan, Xianghua ^{[1
]}

He, Ziyi ^{[1
]}

机构：

[1] Huazhong Agr Univ, Coll Sci, Coll Anim Sci & Technol, Coll Vet Med,State Key Lab Agr Microbiol,Hubei Hon, Wuhan 430070, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2023年 / 380卷

基金：

中国国家自然科学基金;

关键词：

Bacteria; Antibiotic susceptibility testing; Digital enumeration; Droplet; Vibrating capillary; RESISTANCE;

D O I：

10.1016/j.snb.2022.133254

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Antibiotic resistance can lead to multidrug-resistant superbugs and threaten global health, putting forward a high demand for accurate and fast antibiotic susceptibility testing (AST). Conventional AST methods are labor-intensive and time-consuming, far from meeting the requirement for rapid clinical decision-making. Here, we develop a portable vibrating capillary-based droplet platform for precise digital bacteria enumeration and rapid AST. Multi-volume droplets encapsulating bacteria with or without antibiotics are generated on demand, and the elevated fluorescence from resazurin reduction is measured, which corresponds to the growing bacteria. Auto-mated data analysis is achieved by a self-developed image processing program. Multi-volume droplet digital counting allows precise bacteria enumeration over a wide dynamic range spanning more than 4 orders of magnitude. Droplet digital AST of ampicillin against E. coli demonstrates that it is feasible for accurately assessing the minimum inhibitory concentration and investigating the inoculum effect, with the assay time reduced to 5 h. In sum, this method is easy to operate, cost-effective, with high accuracy and short time-to-result, which is highly promising for rapid and precise assessment of antimicrobial susceptibility in resource-limited settings.

引用

页数：8