A novel high-affinity potassium transporter SeHKT1;2 from halophyte Salicornia europaea shows strong selectivity for Na+ rather than K+

被引:7
作者
Haxim, Yakupjan [1 ,2 ,3 ]
Wang, Lei [1 ]
Pan, Zhendong [1 ,3 ]
Fan, Xiaorong [4 ]
Ma, Jinbiao [1 ,2 ,3 ]
机构
[1] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Key Lab Ecol Safety & Sustainable Dev Arid Lands, Urumqi, Peoples R China
[2] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Xinjiang Key Lab Conservat & Utilizat Plant Gene R, Urumqi, Peoples R China
[3] Chinese Acad Sci, Turpan Eremophytes Bot Garden, Turpan, Peoples R China
[4] Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, MOA Key Lab Plant Nutr & Fertilizat Low Middle Rea, Nanjing, Peoples R China
来源
FRONTIERS IN PLANT SCIENCE | 2023年 / 14卷
关键词
Salicornia europaea; salt tolerance; high-affinity K+ transporters; HKT; ion selectivity; SALT TOLERANCE; HKT TRANSPORTERS; SODIUM-TRANSPORT; RICE OSHKT2/1; ARABIDOPSIS; GENE; MECHANISMS; SALINITY; HOMOLOG; CHANNEL;
D O I
10.3389/fpls.2023.1104070
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
High-affinity K+ transporters (HKTs) are known as transmembrane cation transporters and are involved in Na+ or Na+-K+ transport in plants. In this study, a novel HKT gene SeHKT1;2 was isolated and characterized from the halophyte, Salicornia europaea. It belongs to subfamily I of HKT and shows high homology with other halophyte HKT proteins. Functional characterization of SeHKT1;2 indicated that it contributes to facilitating Na+ uptake in Na+-sensitive yeast strains G19, however, cannot rescue the K+ uptake-defective phenotype of yeast strain CY162, demonstrating SeHKT1;2 selectively transports Na+ rather than K+. The addition of K+ along with NaCl relieved the Na+ sensitivity. Furthermore, heterologous expression of SeHKT1;2 in sos1 mutant of Arabidopsis thaliana increased salt sensitivity and could not rescued the transgenic plants. This study will provide valuable gene resources for improving the salt tolerance in other crops by genetic engineering.
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页数:9
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